Abcam, ab50887, Anti-Twist antibody [Twist2C1a]

Size: 100µg
Anti-Twist antibody [Twist2C1a] (ab50887) is a mouse monoclonal antibody detecting Twist in Western Blot . Suitable for Human, Mouse, Rat . - Over 240 publications - Trusted since 2007
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:Twist2C1a,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human TWIST1. The exact immunogen used to generate this antibody is proprietary information.Q15672,
Specificity:Good controls are NIH3T3 and A2058 cell lysates. Given that the immunogen shares 92% homology with Twist2, it is possible that it may cross-react.Although some customers have obtained good results in IHC, we do not recommend this antibody for use in IHC.

Product details:
What is this antibody validated in?
Anti-Twist antibody [Twist2C1a] (ab50887) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.
What is the molecular weight of Twist?
Anti-Twist [Twist2C1a] (ab50887) specifically detects a band for Twist (UniProt: Q15672) at a molecular weight of 21kDa.
Trusted by the scientific community
Anti-Twist [Twist2C1a] (ab50887) was first used in a scientific publication in 2007 and has been cited over 240 times in peer-reviewed journals.
Reviewed by scientists
Anti-Twist [Twist2C1a] (ab50887) has over 25 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from TCS, Storage buffer-pH: 7.4Preservative: 0.05% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Twist protein also known as Twist1 is a basic helix-loop-helix (bHLH) transcription factor. It has a molecular mass of approximately 21 kDa. Twist is widely expressed in mesodermal tissues during embryonic development including the somites and limb buds. In adults its expression continues in areas involved in tissue regeneration and wound healing.
Biological function summary
Twist plays significant roles in embryogenesis and in wound healing processes. It functions as an important regulator of mesenchymal cell differentiation and is part of the bHLH family of transcription factors which often form dimers to bind specific DNA sequences. Twist modulates the transcription of genes involved in cell movement and survival. It acts as an important player in the epithelial-mesenchymal transition (EMT) a process important for development and cellular dynamics.
Pathways
Twist integrates into multiple biological networks including the Wnt and Notch signaling pathways. It interacts with proteins like Β-catenin in the Wnt pathway and Notch receptors impacting cellular proliferation and fate determination. These interactions highlight its role in regulating cellular behavior and influence various processes such as stem cell maintenance and differentiation.
Twist is implicated in cancer and fibrosis. Its overexpression is often observed in aggressive tumors influencing metastasis through its role in EMT. Twist can interact with oncogenic proteins such as MYC in cancer pathways. In fibrosis aberrant Twist activity leads to excessive tissue remodeling and accumulation of connective tissue. Understanding Twist's function and interactions can aid in developing therapeutic strategies targeting these conditions.