Abcam, ab50974, Anti-CENPC antibody [2159C5a]

Size: 50µg
Mouse Monoclonal CENPC antibody. Suitable for Dot, WB, ICC/IF and reacts with Human samples. Cited in 19 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CENPC aa 650-800.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:2159C5a,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WB, DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human CENPC aa 650-800. The exact immunogen used to generate this antibody is proprietary information.Q03188

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4Preservative: 0.05% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The centromere protein C (CENP-C) plays an important mechanical role in the assembly of the kinetochore a protein structure on chromosomes. Alternative names for this protein include MCM21R and Centromere protein C 1. CENP-C acts as a structural platform bringing together specific components needed for chromosome segregation during cell division. Its mass is approximately 140 kDa. CENP-C is expressed in a broad range of cell types especially in dividing cells or tissues with high turnover rates.
Biological function summary
This protein is essential for the proper attachment of chromosomes to spindle microtubules. CENP-C interacts as a core component of the larger constitutive centromere-associated network (CCAN) complex. This complex contributes to the stability and function of the centromere. Additionally centromeres require CENP-C for correct kinetochore assembly ensuring accurate chromosome alignment and separation during mitosis and meiosis.
Pathways
CENP-C contributes to the mitotic spindle assembly checkpoint and the cell cycle pathway. The interaction with other centromere proteins such as CENP-A CENP-B and CENP-H is significant for proper centromere structure and kinetochore attachment. By doing so these interactions facilitate the checkpoint pathways that prevent premature progression into anaphase until all chromosomes are properly aligned.
Alterations in CENP-C levels or functions associate with cancers and chromosomal instability syndromes. In cancer abnormal CENP-C expression can lead to errors in chromosome segregation contributing to aneuploidy. CENP-C's relationship with proteins like CENP-A emphasizes its role in maintaining chromosomal stability linking it to malignancies where such stability is compromised.