Abcam, ab5103, Anti-Histone H3 (citrulline R2 + R8 + R17) antibody

Size: 100µg
Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103) is a rabbit polyclonal antibody detecting Histone H3 in Western Blot, IP, PepArr . Suitable for Human, Mouse, Rat, . - Over 590 publications - Trusted since 2004
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IP, PepArrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:ab5103 detects a 17 kDa band in single lane Western Blot. Peptide inhibition in Western Blot hasn't been processed. Modification specificity is determined by Peptide Array. ab5103 binds strongly to Histone H3 citrulline 2 + 8 + 17 peptide.In our hands, when tested in western blot, this product typically gives a weaker signal in mouse and rat tissue lysates compared to mouse and rat cell lines.From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.  You may also be interested in our alternative recombinant antibody, ab281584.

Product details:
Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103) is a rabbit polyclonal antibody and is validated for use in IP, PepArr and WB.
Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103) was first used in a scientific publication in 2004 and has been cited over 595 times in peer reviewed journals. It's performance in Western blot, immunofluorescence and IHC in human and mouse samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103) has high sensitivity and specificity.
Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103) has 27 independent reviews from customers.
Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103) specifically detects Histone H3 Citrulline-R2, Citrulline-R8, Citrulline-R17 (UniProt ID: P68431; Molecular weight: 16kDa) and is sold in 100 µg selling sizes.
Top cited antibody for this target with >800 citations. This antibody is a key tool in cancer research, particularly in understanding histone citrullination's role in tumorigenesis and is widely used in studies of gene expression, DNA repair and autoimmune diseases like rheumatoid arthritis and lupus, involving protein arginine deiminases (PADs) and NETosis. Histone hypercitrullination is reported to play a role the progression of various cancers, including multiple myeloma, acute myeloid leukaemia, lung cancer and hepatocellular carcinoma.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle