Abcam, ab51052, Anti-Hsc70 antibody [EP1531Y]

Size: 20µL / 100µL / 1mL
Anti-Hsc70 antibody [EP1531Y] (ab51052) is a rabbit monoclonal antibody detecting Hsc70 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 50 publications - Trusted since 2007
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1531Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WB, Flow Cyt (Intra), IP, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-Hsc70 antibody [EP1531Y] (ab51052) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of Hsc70?
Anti-Hsc70 [EP1531Y] (ab51052) specifically detects a band for Hsc70 (UniProt: P11142) at a molecular weight of 71kDa.
Trusted by the scientific community
Anti-Hsc70 [EP1531Y] (ab51052) was first used in a scientific publication in 2007 and has been cited over 50 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Specificity confirmed
The specificity of Anti-Hsc70 antibody [EP1531Y] (ab51052) has been confirmed by Western blot testing in HSPA8 Knockout A431 cells.
Other related products
We have a range of other formats of antibody clone [EP1531Y] also available for your convenience: ab51052, Carrier free -
ab220821
, APC -
ab319289
, PE -
ab319436
, Alexa Fluor® 488 -
ab319570
, Alexa Fluor® 647 -
ab319683
, Alexa Fluor® 594 -
ab319782
, Alexa Fluor® 555 -
ab319923
, Alexa Fluor® 750 -
ab321639
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The heat shock cognate 70 (Hsc70) protein also known as HSPA8 plays an important role in chaperone-mediated cellular processes. It exhibits a molecular weight of approximately 70 kDa. Hsc70 is abundantly expressed in various tissues and organs including the brain muscle and liver. As a member of the heat shock protein family Hsc70 maintains protein homeostasis by preventing protein aggregation and assisting in protein folding.
Biological function summary
Hsc70 participates in several processes beyond protein folding. This includes its involvement in the transport of proteins across cellular membranes and the degradation of misfolded proteins. Hsc70 often works in conjunction with co-chaperones and forms part of large protein complexes such as the chaperone machinery involved in clathrin-mediated endocytosis. It also plays a part in cellular stress responses supporting cell survival under various stress conditions.
Pathways
Hsc70 integrates into fundamental cellular pathways such as the ubiquitin-proteasome system and the ER-associated degradation pathway. These pathways are important for protein quality control where Hsc70 collaborates with other heat shock proteins like Hsp40 to facilitate the correct folding and clearance of proteins. In the context of autophagy Hsc70 mediates the recognition and trafficking of specific substrates to lysosomes for degradation demonstrating its importance in maintaining cellular health through these pathways.
Hsc70 shows a strong connection to neurodegenerative diseases and cancer. Misregulation of Hsc70 activity can result in protein aggregation seen in disorders such as Parkinson's disease. This situation often links Hsc70 to proteins like alpha-synuclein which accumulate abnormally in these diseases. In cancer altered expression of Hsc70 can promote tumor development by hyper-accommodating the stress conditions within the tumor microenvironment. Hsc70's interplay with proteins such as p53 highlights its role in the pathology of tumorigenesis.