Abcam, ab51804, Anti-Mycobacterium L-alanine dehydrogenase antibody [WHO no. IT-46]

Size: 100µL
Mouse Monoclonal DHA antibody. Suitable for ELISA, WB and reacts with Mycobacterium tuberculosis samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing Mycobacterium L-alanine dehydrogenase protein.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:WHO no. IT-46,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mycobacterium tuberculosis,
Applications:ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:Recognizes the 12 aa SAITDADFKAAG (aa 45-56) region of Ald of Mycobacterium tuberculosis.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, 2.9% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Mycobacterium L-alanine dehydrogenase also known as AlaDH is an enzyme that catalyzes the reversible oxidative deamination of L-alanine to pyruvate and ammonia. This reaction involves NAD+ as a coenzyme. The protein has a molecular mass of approximately 46 kDa. AlaDH is expressed in Mycobacterium species including Mycobacterium tuberculosis. It plays an important role in the metabolic processes of these bacteria particularly in maintaining nitrogen balance.
Biological function summary
L-alanine dehydrogenase participates in nitrogen metabolism and is essential for the survival of Mycobacterium in diverse environments. It does not form part of a larger protein complex but functions independently to facilitate the conversion of amino acids. The activity of AlaDH aids in providing energy and building blocks necessary for cell survival especially when the bacteria are under stress or nutrient-limiting conditions.
Pathways
L-alanine dehydrogenase plays an important role in the alanine and aspartate metabolism pathways. These pathways are critical for energy production and biosynthesis in Mycobacterium species. AlaDH connects closely with other enzymes in the amino acid metabolism network influencing processes such as gluconeogenesis and nitrogen assimilation. Its activity affects the levels of related metabolites impacting the balance of these interconnected metabolic pathways.
L-alanine dehydrogenase has relevance in the study of tuberculosis. The enzyme's function and expression can influence the pathogenicity of Mycobacterium tuberculosis. AlaDH may interact with other proteins involved in Mycobacterium virulence making it a potential target for anti-tuberculosis drugs. Researchers investigate its role in mycobacterial persistence within human hosts contributing to the understanding of chronic infection dynamics.