Abcam, ab52866, Anti-alpha Tubulin antibody [EP1332Y] - Loading Control

Size: 20µL / 100µL / 1mL
Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) is a rabbit monoclonal antibody detecting alpha Tubulin in Western Blot, Flow Cytometry, IHC-P, ICC/IF . Suitable for Drosophila melanogaster, Human, Mouse, Pig, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 240 publications - Trusted since 2007
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1332Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human, Pig, Drosophila melanogaster,
Applications:ICC/IF, Flow Cyt (Intra), IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody is expected to recognise most alpha tubulin proteins and not only TUBA4A.

Product details:
Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, WB in drosophila melanogaster, human, mouse, pig, rat samples.
Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) has been cited over 240 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (Anti-TUBA4A antibody) (ab52866) has 28 independent reviews from customers.
alpha Tubulin antibodies are often used as loading controls in Western Blot. Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker has been verified in Western Blot samples and detects a band at 50kDa Molecular weight.
Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) specifically detects alpha Tubulin (UniProt ID: P68366; Molecular weight: 50kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone EP1332Y -
ab216650
Antibody clone EP1332Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 594, PE, Alexa Fluor® 555 (
ab185031
ab190573
ab202272
ab208752
ab275113
Alpha Tubulin (TUBA4A) is a fundamental component of microtubules, which are crucial for maintaining the cytoskeleton and facilitating cell division. Abnormalities in alpha Tubulin are linked to neurodegenerative diseases such as Alzheimer's Disease, Parkinson's Disease, Amyotrophic Lateral Sclerosis, and Huntington's Disease, as well as cancer, highlighting its disease relevance. Post-translational modifications of alpha Tubulin play a significant role in cellular functions and disease mechanisms. Our TUBA4A antibody is a valuable tool for studying these pathways and understanding the molecular basis of these conditions.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb
patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha tubulin also known as DM1A is an important component of the structural unit called microtubules. This protein weighs approximately 50 kDa and acts as a building block in the cytoskeleton. Alpha tubulin is expressed in various cell types across many organisms playing a fundamental role in maintaining cell shape and enabling intracellular transport. Researchers often acetylate alpha tubulin to study post-translational modifications and its effects on microtubule dynamics.
Biological function summary
Alpha tubulin combines with beta tubulin to form the tubulin dimer which is the basic unit of microtubule polymerization. These dimers assemble into microtubule filaments integral in numerous cellular processes. As part of the cytoskeleton network alpha tubulin facilitates roles in mitosis and meiosis providing spindle structures necessary for chromosome separation. Its participation in cell signaling pathways is noteworthy as it interacts with motor proteins like kinesins and dyneins to aid cellular activities.
Pathways
Alpha tubulin holds critical roles in microtubule-associated processes within the cytoskeleton and intracellular transport pathways. It interacts with the MAP (microtubule-associated protein) family which modulates microtubule stability and impacts cell division and trafficking. Moreover the microtubule pathway involves proteins like tau which bind to stabilize microtubules and are implicated in cellular dynamics and signaling.
Alpha tubulin is relevant in neurodegenerative diseases such as Alzheimer's disease and cancer. Aberrant modifications or mutations in alpha tubulin or associated proteins like tau can disrupt microtubule stability contributing to pathological conditions. In cancer altered expression of alpha tubulin affects cell division leading to tumor progression and chemoresistance. Researchers study alpha tubulin using techniques such as immunofluorescence with Alexa fluor 555 to understand its role in disease better.