Abcam, ab52947, Anti-Heme Oxygenase 1 antibody [EP1391Y]

Size: 100µL / 1mL
Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947) is a rabbit monoclonal antibody detecting Heme Oxygenase 1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P . Suitable for Human, Mouse . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 110 publications - Trusted since 2007
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1391Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IHC-P, Flow Cyt (Intra), IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human, Mouse samples.
What is the molecular weight of Heme Oxygenase 1?
Anti-Heme Oxygenase 1 [EP1391Y] (ab52947) specifically detects a band for Heme Oxygenase 1 (UniProt: P09601) at a molecular weight of 33kDa.
Trusted by the scientific community
Anti-Heme Oxygenase 1 [EP1391Y] (ab52947) was first used in a scientific publication in 2007 and has been cited over 110 times in peer-reviewed journals.
Reviewed by scientists
Anti-Heme Oxygenase 1 [EP1391Y] (ab52947) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Specificity confirmed
The specificity of Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947) has been confirmed by Western blot testing in HMOX1 Knockout A549 cell line,
ab269503
Other related products
We have a range of other formats of antibody clone [EP1391Y] also available for your convenience: ab52947, Carrier free -
ab219360
, PE -
ab305659
, APC -
ab305660
, HRP -
ab305661
, Alexa Fluor® 488 -
ab307765
, Alkaline Phosphatase -
ab308920
, Alexa Fluor® 594 -
ab310664
, Alexa Fluor® 555 -
ab312195
, Alexa Fluor® 568 -
ab312683
, Alexa Fluor® 750 -
ab321675
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Heme Oxygenase 1 also known as HO-1 or HMOX1 is an enzyme that plays an important mechanistic role in heme catabolism. It catalyzes the degradation of heme into biliverdin carbon monoxide and free iron. This process involves the cleavage of the heme ring. HO-1 has a molecular weight of approximately 32 kDa. It is widely expressed in numerous tissues but is especially abundant in the liver and spleen. Its expression is induced by heme and other stress stimuli such as heavy metals cytokines and reactive oxygen species.
Biological function summary
Heme Oxygenase 1 serves important protective functions in the body. It is not part of a larger complex but its products such as carbon monoxide and biliverdin have their own biological activities. Carbon monoxide produced by HO-1 has antiflammatory properties and can modulate apoptotic pathways. Biliverdin is reduced to bilirubin which acts as an antioxidant. The enzyme therefore directly influences cellular stress responses and maintains cellular homeostasis through these processes.
Pathways
Heme Oxygenase 1 is integrally involved in oxidative stress response and heme metabolism. It participates in the cellular response to oxidative damage by reducing oxidative stress and promoting cytoprotection. Through its heme degradation activity it is connected with the synthesis of biologically active molecules like bilirubin and carbon monoxide. Heme Oxygenase 1 activity is related to other proteins in oxidative stress pathways such as Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) which regulates its expression and globins which are sources of heme for HO-1 activity.
Heme Oxygenase 1 has been linked to conditions like cardiovascular diseases and neurodegenerative disorders. Its expression can attenuate the severity of atherosclerosis where oxidative stress is an important factor. In neurodegenerative diseases HO-1's antioxidant properties may provide neuroprotection by mitigating oxidative damage. The protein's interactions with inflammatory cytokines such as Interleukin-6 and tumor necrosis factor-alpha influence its activity in these disease contexts.