Abcam, ab53942, Anti-Glutathione S Transferase mu antibody

Size: 50µL
Goat Polyclonal GSTM1 antibody. Suitable for IP, WB, ICC/IF and reacts with Mouse, Rat, Hamster, Human, Rabbit samples. Cited in 12 publications. Immunogen corresponding to Native Full Length Protein corresponding to Rat Gstm1.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rabbit, Rat, Mouse, Hamster, Human,
Applications:IP, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Rat Gstm1.P04905,
Specificity:ab53942 is specific for Glutathione S Transferase (GST) mu. It reacts with subtype 3-3 (M1) and subtype 4-4 (M2).

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Purification notes-IgG fraction was prepared from the antisera using the caprylic acid precipitation method., Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, 0.7% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glutathione S-transferase mu 1 also known as GSTM1 is an important enzyme involved in detoxification processes. This enzyme belongs to the glutathione S-transferases family which plays an important role in catalyzing the conjugation of glutathione to various electrophilic compounds. GSTM1 has a molecular mass of approximately 26 kDa and is primarily expressed in the liver but it is also present in other tissues such as the kidney and erythrocytes. GSTM1's active site involves a cysteine residue that facilitates the transfer of glutathione to potential harmful substances aiding in their subsequent excretion from the body.
Biological function summary
The enzyme contributes to maintaining cellular homeostasis by neutralizing toxins and protecting against oxidative stress. GSTM1 is part of a larger family of enzymes each with specific substrate affinities but all contributing to the detoxification of xenobiotics and carcinogens. This process is critically important to protect cells from environmental and oxidative damage. The enzyme highly expresses in liver tissue highlights its role in processing and eliminating harmful substances from the body.
Pathways
GSTM1 plays a significant role in the xenobiotic metabolism pathway where it participates in the detoxification and clearance of various xenobiotic substances. Another important pathway involving GSTM1 is the glutathione metabolism pathway. Within these pathways GSTM1 interacts with other glutathione S-transferases and proteins such as cytochrome P450s which also contribute to the metabolism of endogenous and exogenous compounds. The interaction with these proteins highlights its participation in a coordinated defense mechanism against chemical insults.
An absence or deficiency of GSTM1 is associated with an increased risk of conditions such as asthma and certain cancers particularly bladder cancer. The lack of GSTM1's detoxifying activity may increase susceptibility to environmental toxins and carcinogens. This enzyme together with other glutathione S-transferases plays a role in mitigating oxidative stress related to these conditions. Through its interactions with cytochrome P450 enzymes GSTM1 influences metabolic activation and deactivation of various potential carcinogens highlighting its relevance in disease prevention strategies.