Abcam, ab5417, Anti-Rhodopsin antibody [1D4]

Size: 100µL
Anti-Rhodopsin antibody [1D4] (ab5417) is a mouse monoclonal antibody detecting Rhodopsin in Western Blot, IP, IHC-P, IHC-Fr, ICC/IF, ELISA . Suitable for Amphibian, Cow, Human, Mouse, Rat, Zebrafish . - Over 100 publications - Trusted since 2003
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:1D4,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Human, Amphibian, Cow, Zebrafish, Rat,
Applications:IP, IHC-Fr, ELISA, IHC-FoFr, IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Epitope:The epitope for this antibody has been localized to the C-terminal nine amino acids of bovine rhodopsin known as the 1D4 epitope.,
Specificity:ab5417 detects Rhodopsin from human and bovine retinal samples. Data from Yin J et al., 2012 (PMID 22743318) indicates that in Zebrafish ab5417 appears to recognize Red Opsin rather than Rhodopsin.

Product details:
Product Specifications
Anti-Rhodopsin antibody [1D4] (ab5417) is a mouse monoclonal antibody and is validated for use in ELISA, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, IP, WB in amphibian, cow, human, mouse, rat, zebrafish samples.
Anti-Rhodopsin antibody [1D4] (ab5417) specifically detects Rhodopsin (UniProt ID: P08100; Molecular weight: 39kDa) and is sold in 100 µL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-Rhodopsin antibody [1D4] (ab5417) has high sensitivity and specificity.
Anti-Rhodopsin antibody [1D4] (ab5417) has been cited over 108 times in peer reviewed journals and is trusted by the scientific community.
Anti-Rhodopsin antibody [1D4] (ab5417) has 4 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rhodopsin also known as visual purple is a protein found in the photoreceptor cells of the retina. It has a mass of approximately 39 kDa. This protein acts as a light-sensitive receptor playing an essential role in the function of rod cells. Rhodopsin is expressed predominantly in the rod outer segment where it transduces light signals. The crystallized form of this receptor referred to as PDB ID: 1D4 has helped researchers study its structure in detail.
Biological function summary
Rhodopsin enables night vision by absorbing photons and activating a G-protein-coupled receptor (GPCR) cascade. Rhodopsin is an integral part of a protein complex within the phototransduction pathway where it works closely with transducin to amplify the visual signal. It initiates the conversion of the photon into an electrical signal. Rhodopsin's role is triggering a structural change in response to light converting from its inactive form to a signaling state.
Pathways
Rhodopsin is deeply involved in the phototransduction and retinoid cycle pathways. Once it absorbs light the transducin it binds causes the activation of a cascade that ultimately results in hyperpolarization of the rod cells. This network of interactions also involves arrestin and recoverin which work together to regulate rhodopsin activity and signal termination. The process ensures precise response and recovery of the visual signal in low-light conditions.
Rhodopsin mutations and malfunctions connect to retinitis pigmentosa and congenital stationary night blindness. Mutations can lead to improper folding or misregulation of the protein impacting its function. Visual cycle proteins like RPE65 are often affected alongside rhodopsin in retinitis pigmentosa. These associations stress rhodopsin's importance in maintaining healthy retinal function and its link to eye diseases.