Abcam, ab54481, Anti-PGC1 alpha + beta antibody - BSA and Azide free

Size: 50µL
Anti-PGC1 alpha + beta antibody - BSA and Azide free (ab54481) is a rabbit polyclonal antibody provided in a PBS only buffer for easy conjugation detecting PGC1 alpha + beta in Western Blot . Suitable for Human, Mouse . - BSA, sodium azide, and glycerol-free for easy conjugation - Over 540 publications - Trusted since 2007
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PPARGC1A aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9UBK2,
Specificity:The immunogen sequence for this antibody was from PGC1 alpha, and is 78% homologous with PGC1 beta (Uniprot: Q86YN6), which this antibody also detects.

Product details:
What is this antibody validated in?
Anti-PGC1 alpha + beta antibody - BSA and Azide free (ab54481) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse samples.
What is the molecular weight of PGC1 alpha + beta?
Anti-PGC1 alpha + beta  - BSA and Azide free (ab54481) specifically detects a band for PGC1 alpha + beta (UniProt: Q9UBK2) at a molecular weight of 92kDa.
Trusted by the scientific community
Anti-PGC1 alpha + beta  - BSA and Azide free (ab54481) was first used in a scientific publication in 2007 and has been cited over 540 times in peer-reviewed journals.
Reviewed by scientists
Anti-PGC1 alpha + beta  - BSA and Azide free (ab54481) has over 20 independent reviews from customers.
This product may be covered by one or more of the following U.S. Patents.
6,166,192
6,426,411
6,525,178
6,872,391
6,908,987
7,250,283
7,091,006
7,354,738
8,105,774
8,445,233

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PGC1 alpha and beta also known as Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha (PGC-1α) and 1-beta (PGC-1β) are transcriptional coactivators with molecular weights of approximately 91 kDa and 102 kDa respectively. These proteins are important in regulating energy metabolism and are highly expressed in tissues with high energy demand such as skeletal muscle heart and brown adipose tissue. The proteins interact with nuclear hormone receptors and various transcription factors to control the expression of genes involved in energy homeostasis.
Biological function summary
PGC1 alpha and beta activate and coordinate the expression of genes involved in mitochondrial biogenesis and oxidative phosphorylation. They do not form a complex themselves but work with other transcriptional regulators to enhance gene expression. These proteins influence the metabolic processes of cells regulating the conversion of energy substrates into usable cellular energy. They are critical in adapting to different physiological conditions like exercise and fasting by modulating the capacity for energy production.
Pathways
PGC1 alpha and beta integrate signals from various pathways like the PPAR and AMPK pathways which are significant for energy balance and metabolism. PGC1 alpha particularly interacts with proteins such as NRF1 and NRF2 to promote mitochondrial function. Through these pathways PGC1 coactivators help regulate metabolism by altering the transcriptional activity of genes involved in energy expenditure and metabolic adaptation.
PGC1 alpha and beta have significant implications in conditions such as obesity and diabetes. Dysregulation of these proteins affects the balance of energy metabolism leading to metabolic disorders. PGC1 alpha in particular interacts with proteins like SIRT1 which influence insulin sensitivity and metabolic rate thereby playing a role in the development and progression of these diseases. The modulation of PGC1 activity has potential therapeutic implications for managing metabolic disorders.