Abcam, ab55152, Anti-MHC Class II antibody [6C6]

Size: 100µg
Anti-MHC Class II antibody [6C6] (ab55152) is a mouse monoclonal antibody detecting MHC Class II in Western Blot, Flow Cytometry, IHC-P, ICC/IF. Suitable for Human. -Carrier Free -Produces clear membrane and cytoplasmic staining in antigen-presenting cells. -Cited in over 30 publications -Trusted since 2007
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:6C6,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, Flow Cyt, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human HLA-DPB1.P04440

Product details:
Anti-MHC Class II antibody [6C6] (ab55152) is a House Mouse Monoclonal antibody and is validated for use in Flow Cyt, ICC/IF, IHC-P, WB.
Anti-MHC Class II antibody [6C6] (ab55152) has been cited over 33 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality validation processes ensure Anti-MHC Class II antibody [6C6] (ab55152) has high sensitivity and specificity.
Anti-MHC Class II antibody [6C6] (ab55152) specifically detects MHC Class II (UniProt ID: P04440; Molecular weight: 26kDa) and is sold in 100 ug selling sizes.
MHC Class II molecules are primarily expressed on professional antigen-presenting cells (APCs) like dendritic cells, macrophages, and B cells. In oncology, MHC Class II molecules play a crucial role by presenting exogenous antigens to CD4+ T cells, which is essential for initiating and regulating the immune response against tumors.
This product was changed from ascites to tissue culture supernatant on 13
Feb 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MHC Class II also known as MHC II or human leukocyte antigen (HLA) class II is a protein complex with a molecular mass generally around 55 kDa. This protein is mainly expressed on the surface of antigen-presenting cells such as dendritic cells macrophages and B cells. It plays an important role in the immune system by presenting extracellular antigens to T helper cells. MHC II molecules are composed of two polypeptide chains alpha and beta which associate to form a heterodimer on the cell surface.
Biological function summary
MHC Class II molecules facilitate the immune system's recognition and response to pathogens by presenting processed antigenic peptides to CD4+ T lymphocytes. This activity involves their assembly within the endosomal-lysosomal system where they bind peptides derived from endocytosed proteins. MHC II is part of the major histocompatibility complex which includes other related molecules such as MHC Class I (HLA class I). The binding and presentation of peptides are central for the coordination of adaptive immune responses enhancing pathogen clearance and maintaining immunological memory.
Pathways
MHC Class II operates within the antigen processing and presentation pathway important for adaptive immunity. Its interaction with CD4+ T cells stimulates downstream signaling pathways leading to T cell activation and differentiation. Related proteins in the antigen presentation pathway include invariant chain (Ii) which guides MHC II to the endosome and HLA-DM which facilitates peptide loading. Together these proteins and pathways work to ensure effective immune surveillance and response.
Alterations in MHC Class II expression or function are associated with autoimmune diseases such as rheumatoid arthritis and type 1 diabetes. In these conditions improper antigen presentation may lead to an inappropriate immune response against self-proteins. The involvement of MHC II in disease is often linked to specific HLA alleles which vary among individuals and influence susceptibility to autoimmune disorders. Additionally the abnormal function of connected proteins like cytokines from activated T helper cells can exacerbate these immune conditions.