Abcam, ab5659, Anti-PDE6 alpha antibody

Size: 100µg
Rabbit Polyclonal PDE6 alpha antibody. Suitable for IHC-P, WB and reacts with Human, Sheep samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human PDE6A aa 1-50.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Sheep, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PDE6A aa 1-50. The exact immunogen used to generate this antibody is proprietary information.P16499

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Phosphodiesterase 6 alpha (PDE6 alpha) is an enzyme that plays a significant role in the phototransduction pathway. It is also known as PDE6A and has a molecular mass of approximately 100 kDa. This enzyme is highly expressed in the rod and cone cells of the retina acting as a component in the conversion of light signals into visual information. PDE6 alpha hydrolyzes cyclic GMP into 5'-GMP decreasing the intracellular levels of cGMP. This action is critical in regulating the conductance of ion channels in photoreceptor cells.
Biological function summary
PDE6 alpha functions within a larger enzyme complex known as the PDE6 holoenzyme. This complex consists of multiple subunits including the PDE6 beta and PDE6 gamma subunits which form the catalytic core. PDE6 alpha contributes to the regulation of cGMP levels which is essential for maintaining the electrical response of photoreceptors to light. The precise action of hydrolyzing cGMP mediates the phototransduction cascade that eventually leads to visual perception.
Pathways
With regard to pathways in cellular function PDE6 alpha is an important player in the phototransduction and visual signal transduction pathways. Within the phototransduction pathway PDE6 alpha interacts closely with other proteins like rhodopsin and transducin. These proteins activate PDE6 alpha upon light exposure initiating the cascade that results in hyperpolarization of the photoreceptor cell membrane. The role of PDE6 alpha in managing cGMP concentration positions it as a major component in these pathways.
Mutations in the genes encoding PDE6 alpha have been associated with retinitis pigmentosa and congenital stationary night blindness. Retinitis pigmentosa a progressive degenerative disease of the retina often involves the dysfunction of photoreceptor cells where altered PDE6 alpha function impacts the phototransduction process. Additionally PDE6 alpha's involvement in these disorders also connects it to proteins such as arrestin and recoverin which further influence the progression of these visual impairments.