Abcam, ab57461, Anti-ARID1B antibody [2D2]

Size: 100µg
Anti-ARID1B antibody [2D2] (ab57461) is a mouse monoclonal antibody detecting ARID1B in Western Blot, Flow Cytometry, IHC-P, ICC/IF . Suitable for Human . - KO validated for confirmed specificity - Over 20 publications - Trusted since 2007
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:2D2,
Isotype:IgG2b,
Light chain type:kappa,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, Flow Cyt, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ARID1B aa 1350-1500. The exact immunogen used to generate this antibody is proprietary information.Q8NFD5

Product details:
What is this antibody validated in?
Anti-ARID1B antibody [2D2] (ab57461) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of ARID1B?
Anti-ARID1B [2D2] (ab57461) specifically detects a band for ARID1B (UniProt: Q8NFD5) at a molecular weight of 236kDa.
Trusted by the scientific community
Anti-ARID1B [2D2] (ab57461) was first used in a scientific publication in 2007 and has been cited over 20 times in peer-reviewed journals.
Specificity confirmed
The specificity of Anti-ARID1B antibody [2D2] (ab57461) has been confirmed by Western blot testing in ARID1B Knockout HCT 116 cells.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ARID1B also known as BAF250b is a component of the SWI/SNF chromatin remodeling complex. This protein has a mass of approximately 250 kDa. ARID1B interacts with DNA to regulate gene expression by modifying the chromatin structure making it more accessible for transcription machinery. It is expressed in various tissues including the brain heart and skeletal muscle suggesting a role in diverse physiological processes.
Biological function summary
The protein participates in the regulation of transcription by altering the chromatin architecture. As part of the SWI/SNF complex ARID1B works alongside other proteins to control which parts of the genome are accessible for transcription. Its activity influences numerous cellular events like cell differentiation and proliferation. The SWI/SNF complex plays a significant role in embryonic development and tumor suppression.
Pathways
ARID1B affects several critical biological processes such as Wnt signaling and cell cycle regulation. The protein interacts with beta-catenin in the Wnt pathway affecting gene expression and cell fate decisions. Additionally ARID1B modulates the cell cycle directly by influencing the expression of cyclins and cyclin-dependent kinases proteins essential for cell cycle progression.
Mutations in the ARID1B gene have associations with neurodevelopmental conditions like Coffin-Siris syndrome and intellectual disabilities. The protein's malfunction can disrupt normal brain development through pathways involving CREBBP a known coactivator in transcription. Additionally ARID1B alterations appear in certain cancers often resulting in abnormal proliferation and differentiation in affected cells highlighting the importance of its regulatory functions in maintaining cellular health.