Abcam, ab57602, Anti-Mitofusin 2 + Mitofusin 1 antibody [3C9]

Size: 100µg
Anti-Mitofusin 2 + Mitofusin 1 antibody [3C9] (ab57602) is a mouse monoclonal antibody detecting Mitofusin 1 in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Cynomolgus monkey, Human, Mouse, Rat . - Over 200 publications - Trusted since 2007
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:3C9,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human, Rat, Cynomolgus monkey, Mouse,
Applications:WB, IP, IHC-P, ICC/IF, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human MFN1.Q8IWA4,
Specificity:The immunogen used for this product shares 63% homology with MFN2.ab57602 binds to Mitofusin 1 (*Mfn1*) and mitofusin 2 (Mfn2) in western blot.

Product details:
What is this antibody validated in?
Anti-Mitofusin 2 + Mitofusin 1 antibody [3C9] (ab57602) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Cynomolgus monkey, Human, Mouse, Rat samples.
What is the molecular weight of Mitofusin 1?
Anti-Mitofusin 2 + Mitofusin 1 [3C9] (ab57602) specifically detects a band for Mitofusin 1 (UniProt: Q8IWA4) at a molecular weight of 84kDa.
Trusted by the scientific community
Anti-Mitofusin 2 + Mitofusin 1 [3C9] (ab57602) was first used in a scientific publication in 2007 and has been cited over 200 times in peer-reviewed journals.
Reviewed by scientists
Anti-Mitofusin 2 + Mitofusin 1 [3C9] (ab57602) has over 10 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Purification notes-Purified from TCS, Storage buffer-pH: 7.4Constituents: 91% Water, 8% Sodium chloride, 0.6% Disodium hydrogenorthophosphate, 0.2% Potassium chloride, 0.2% Potassium phosphate monobasic, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The mitofusins coordinate mitochondrial network maintenance contributing to the stabilization of cellular energy supply. Mitofusins 1 and 2 participate in forming a complex that ensures mitochondrial integrity and function. By facilitating the fusion of mitochondria these proteins help maintain mitochondrial DNA stability and bioenergetics which are vital for efficient cellular metabolism. These tasks are important for cells that undergo high energy demand thereby ensuring proper ATP production.
Pathways
The mitofusins participate in mitochondrial fusion pathways which are essential for cellular energy metabolism and apoptosis regulation. Mfn1 and Mfn2 along with other proteins like OPA1 ensure mitochondrial structure and function are maintained during these processes. This activity impacts pathways such as the PINK1/Parkin-mediated mitophagy pathway which is important for maintaining healthy mitochondria by eliminating damaged ones.
Mitofusins demonstrate significance in conditions like Charcot-Marie-Tooth disease and mitochondrial dysfunction syndromes. Mutations in Mfn2 link directly to Charcot-Marie-Tooth disease type 2A leading to neuropathy. Additionally impaired mitofusins' function associates with metabolic disorders as mitochondrial dynamics play a role in regulating glucose homeostasis. Understanding their roles further clarifies the interactions with other proteins such as Drp1 contributing to metabolic flexibility and neurodegeneration pathways.
Mitofusin 1 (Mfn1) and Mitofusin 2 (Mfn2) are key components involved in mitochondrial dynamics. These proteins often referred to as mitofusins facilitate the fusion of mitochondrial membranes. The molecular weight of Mfn1 is approximately 84 kDa while Mfn2 is around 86 kDa. Both mitofusins are ubiquitous with expression in diverse tissues particularly in metabolically active cells such as in the heart muscle and brain. Mfn2 is sometimes called 2c-10<3c-9 highlighting its genetic coding. Mitofusin 2 also plays a unique role in interactions beyond membrane fusion.