Abcam, ab5790, Anti-PAX6 antibody

Size: 100µg
Anti-PAX6 antibody (ab5790) is a rabbit polyclonal antibody detecting PAX6 in Western Blot, IP, ICC/IF . Suitable for Human, Monkey, Mouse, Rat . - Over 120 publications - Trusted since 2003
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human, Monkey, Mouse,
Applications:WB, ICC/IF, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Mouse Pax6 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.P63015,
Specificity:This antibody does not detect bovine PAX6 by Western blot.We have had mixed results for use of this antibody in IHC-Fr. Thus, we are removing IHC-Fr as a guaranteed application and welcome any feedback from customers who have used this antibody in IHC-Fr.

Product details:
What is this antibody validated in?
Anti-PAX6 antibody (ab5790) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Monkey, Mouse, Rat samples.
What is the molecular weight of PAX6?
Anti-PAX6 (ab5790) specifically detects a band for PAX6 (UniProt: P63015) at a molecular weight of 50.6kDa.
Trusted by the scientific community
Anti-PAX6 (ab5790) was first used in a scientific publication in 2003 and has been cited over 120 times in peer-reviewed journals.
Reviewed by scientists
Anti-PAX6 (ab5790) has over 20 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The PAX6 protein also known as paired box 6 is a transcription factor involved in the development of eyes and neural tissues. It consists of a paired domain and a homeodomain collectively giving a molecular weight of approximately 48 kDa. This protein is highly expressed in developing ocular and central nervous tissues where it controls the expression of genes necessary for proper development. PAX6 is a widely studied marker in developmental biology due to its critical role in cellular differentiation and patterning processes.
Biological function summary
PAX6 acts as an important regulator in eye and neural development functioning alone and as part of larger complexes with other transcription factors. This role ensures the correct formation of ocular structures including the lens retina and cornea and neural elements by activating downstream targets important for cell fate decisions. The PAX6 protein intricately influences the genetic circuits that guide stem cell differentiation highlighting its importance in embryogenesis and organogenesis.
Pathways
PAX6 is pivotal in the Wnt signaling pathway and retinoic acid signaling. Its interaction within the Wnt pathway orchestrates vital developmental processes including those of eye morphogenesis and brain patterning. The protein collaborates with other transcriptional regulators such as SOX2 and EYA1 within these pathways facilitating the complex network of signals necessary for organ development and cellular identity specification.
PAX6 mutations have strong associations with aniridia and congenital eye malformations. Mutations in PAX6 disrupt the transcriptional regulation required for eye development often resulting in severe vision defects and structural anomalies. The intracacy of its involvement extends to disorders like Wilms' tumor wherein its altered expression or function in conjunction with proteins such as WT1 may contribute to oncogenesis. Scientists frequently use PAX6 staining to investigate these conditions and better understand their molecular underpinnings.