Abcam, ab58522, Anti-Hsp20 (phospho S16) antibody

Size: 100µg
Rabbit Polyclonal Hsp20 phospho S16 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human HSPB6 phospho S16 aa 1-100.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human HSPB6 phospho S16 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.O14558

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-The antibody against non-phosphopeptide was removed by chromatographyusing non-phosphopeptide corresponding to the phosphorylation site., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Hsp20 also known as Heat Shock Protein 20 or HspB6 is a small heat shock protein with a molecular weight of approximately 20 kDa. It functions by binding to partially unfolded proteins preventing them from aggregating and assisting in their proper folding. Hsp20 achieves this through its chaperone activity similar to other small heat shock proteins. This protein is expressed ubiquitously with high levels found in cardiac and skeletal muscles as well as in the brain and placenta.
Biological function summary
The small heat shock protein family which includes Hsp20 plays a critical role in maintaining cellular proteostasis under stress conditions. Hsp20 does not typically form large complexes though it may oligomerize under certain conditions to exert its protective functions. It interacts with cellular structures and proteins to maintain cell integrity and function. Hsp20 can modulate actin filament dynamics influencing cellular motility and smooth muscle relaxation.
Pathways
The Hsp20 protein is actively involved in several key regulatory pathways. It plays a role in the stress response pathway and is engaged in the cardiomyocyte signaling network. Hsp20 interacts closely with proteins such as Akt and PKA which are involved in the signaling cascades that govern cell survival and response to stress. Phosphorylation of Hsp20 by PKA contributes to its protective functions especially in cardiac tissues.
Alterations in Hsp20 expression or function can impact the development of cardiac diseases and neurodegenerative disorders. The protein is associated with cardiomyopathies where its protective role is important in managing stress in cardiac cells. Furthermore changes in Hsp20 have implications for Alzheimer's disease due to its interactions with tau protein. Enhanced understanding of Hsp20's mechanisms might lead to better therapeutic strategies for these diseases.