Abcam, ab58989, Anti-CD9 antibody [TS9] - BSA and Azide free

Size: 100µg
Anti-CD9 antibody [TS9] - BSA and Azide free (ab58989) is a mouse monoclonal antibody provided in a PBS only buffer for easy conjugation detecting CD9 in Western Blot . Suitable for Human . - KO validated for confirmed specificity - BSA, sodium azide, and glycerol-free for easy conjugation - Over 10 publications - Trusted since 2007
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:TS9,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:Yes,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Cell preparation containing CD9 protein. The exact immunogen used to generate this antibody is proprietary information.P21926,
Specificity:Recognises the p24 antigen, a 24 kda protein.

Product details:
What is this antibody validated in?
Anti-CD9 antibody [TS9] - BSA and Azide free (ab58989) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Human samples.
What is the molecular weight of CD9?
Anti-CD9 [TS9] - BSA and Azide free (ab58989) specifically detects a band for CD9 (UniProt: P21926) at a molecular weight of 24kDa.
Trusted by the scientific community
Anti-CD9 [TS9] - BSA and Azide free (ab58989) was first used in a scientific publication in 2007 and has been cited over 10 times in peer-reviewed journals.
Specificity confirmed
The specificity of Anti-CD9 antibody [TS9] - BSA and Azide free (ab58989) has been confirmed by Western blot testing in CD9 Knockout HeLa cell line,
ab255375

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.3 - 7.5Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD9 also known as motility-related protein-1 (MRP-1) is a transmembrane glycoprotein with a molecular weight of approximately 24 kDa. This protein is part of the tetraspanin family and shows widespread expression throughout different tissues including hematopoietic and non-hematopoietic cells. CD9 participates in numerous cellular processes by interacting with integrins and other cell surface receptors facilitating the organization of molecular complexes within the cell membrane.
Biological function summary
The CD9 protein is integral to cell adhesion migration and fusion. It forms complexes with other tetraspanins and proteins like EWI-2 and EWI-F contributing to cellular signaling and membrane compartmentalization. Additionally CD9 plays an important role in the formation and secretion of exosomes tiny vesicles emitted by cells that mediate cell-to-cell communication. These exosomes can be analyzed using tools like exosome assays and detection kits that target CD9 proteins.
Pathways
CD9 is involved in the regulation of immune response and cell morphology. It participates in pathways such as the integrin signaling pathway and the phosphatidylinositol 3-kinase (PI3K) signaling pathway. CD9 modulates these interactions by associating with proteins such as integrins and other members of the tetraspanin family influencing cellular movement and proliferation.
CD9 has connections to various conditions including cancer and infectious diseases. In cancer CD9 can influence tumor progression and metastasis while its altered expression levels have been associated with different cancer types. CD9 interacts with proteins such as integrins and CD81 in these contexts affecting cellular adhesion and migration mechanisms. In infectious diseases CD9 is involved in viral entry processes as some viruses utilize CD9 and its associated tetraspanins for entry into host cells.