Abcam, ab59479, Anti-CD63 antibody [TS63] - BSA and Azide free

Size: 100µg
Anti-CD63 antibody [TS63] - BSA and Azide free (ab59479) is a mouse monoclonal antibody provided in a PBS only buffer for easy conjugation detecting CD63 in Western Blot, Flow Cytometry, IHC-P, ICC/IF . Suitable for Human . - BSA, sodium azide, and glycerol-free for easy conjugation - Over 250 publications - Trusted since 2007
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:TS63,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, IHC-P, WB, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Cell preparation containing CD63 protein. The exact immunogen used to generate this antibody is proprietary information.P08962,
Specificity:Recognises the LIMP antigen, a 30-60 kda (smear) protein.

Product details:
What is this antibody validated in?
Anti-CD63 antibody [TS63] - BSA and Azide free (ab59479) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human samples.
What is the molecular weight of CD63?
Anti-CD63 [TS63] - BSA and Azide free (ab59479) specifically detects a band for CD63 (UniProt: P08962) at a molecular weight of 26kDa.
Trusted by the scientific community
Anti-CD63 [TS63] - BSA and Azide free (ab59479) was first used in a scientific publication in 2007 and has been cited over 250 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purification technique-Ion exchange chromatography, Purification notes-Sterile-filtered through 0.22 µm and treated to remove endotoxins., Storage buffer-pH: 7.3 - 7.5Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD63 is a protein commonly known as LAMP-3 a member of the tetraspanin family with a molecular weight of approximately 25-65 kDa depending on post-translational modifications such as glycosylation. It resides mainly on the membrane of intracellular vesicles such as lysosomes and platelets. CD63 also appears on the surface of activated cells particularly in immune cells. Researchers often use CD63 as a marker in assays including CD63 western blot techniques due to its distinct expression patterns.
Biological function summary
CD63 participates in various cellular processes including membrane trafficking cell adhesion and signal transduction. It associates with integrins and other tetraspanins forming complexes that influence cellular adhesion and migration. Through its role in exosome formation CD63 contributes significantly to intercellular communication by facilitating the transfer of proteins lipids and RNA between cells.
Pathways
CD63 plays a role in pathways linked to cell proliferation and migration and immune response regulation. Within the integrin-mediated signaling pathway CD63 interacts with proteins such as integrins to promote cell adhesion and migration. CD63 also links to vesicle trafficking pathways collaborating with proteins involved in exocytosis and endocytosis which affect cell membrane restructuring and intracellular transport.
CD63 shows connections with cancer and immune system disorders. Its role in promoting cell adhesion and migration links it with cancer metastasis including melanoma and breast cancer where CD63 expression levels often rise. Additionally CD63 associates with allergic reactions due to its involvement in histamine release where it impacts immune proteins like FcεRI which participate in allergy pathogenesis.