Abcam, ab6002, Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade

Size: 100µg
Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) is a mouse monoclonal antibody detecting Histone H3 in  IP, IHC- Wmt, ICC/IF, ELISA, ChIP . Suitable for Cow, Human, Mouse . - Over 980 publications - Trusted since 2004
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:mAbcam 6002,
Isotype:IgG3,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human, Mouse, Cow,
Applications:ELISA, IHC - Wmt, ICC/IF, ChIPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody is specific for histone H3 tri-methylated at K27. The antibody is blocked in Western blot by tri methyl K27 peptide and slightly by di methyl K27 peptide (there is <12% cross reactivity with di methyl K27 as determined by ELISA). It is not blocked by mono methyl K4, di methyl K4, tri methyl K4, mono methyl K9, di methyl K9, tri methyl K9, mono methyl K27 or unmodified K27 peptides (see the peptide blocking assay blot below).

Product details:
Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) is a mouse monoclonal antibody and is validated for use in ChIP, ELISA, ICC/IF, IHC- Wmt and WB.
Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) was first used in a scientific publication in 2002 and has been cited over 982 times in peer reviewed journals. It's performance in ChIP and Western Blot in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) has high sensitivity and specificity.
Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) has 70 independent reviews from customers.
Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) specifically detects Histone H3 Tri Methyl-K27 (UniProt ID: P68431; Molecular weight: 16kDa) and is sold in 100 µg selling sizes.
Top cited antibody for this target with >1000 citations. A highly cited Histone H3 tri methyl K27 antibody, essential for studying epigenetic modifications and gene silencing. This antibody is crucial in chromatin remodeling research, particularly in understanding the role of H3K27me3 in transcription regulation. It is widely used in studies of Polycomb repressive complexes and histone methylation. Histone H3 lysine 27 methylation (H3K27) is a key marker associated with heterochromatin formation and widely used as a marker for transcriptional repression.
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This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 6.97% L-Arginine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle