Abcam, ab60704, Anti-NeuroD1 antibody [3H8] - BSA and Azide free

Size: 100µg
Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704) is a mouse monoclonal antibody provided in a PBS only buffer for easy conjugation detecting NeuroD1 in Western Blot, Flow Cytometry, IHC-P . Suitable for Human . - BSA, sodium azide, and glycerol-free for easy conjugation - Over 50 publications - Trusted since 2007
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:3H8,
Isotype:IgG2a,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-P, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human NEUROD1 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.Q13562

Product details:
What is this antibody validated in?
Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human samples.
What is the molecular weight of NeuroD1?
Anti-NeuroD1 [3H8] - BSA and Azide free (ab60704) specifically detects a band for NeuroD1 (UniProt: Q13562) at a molecular weight of 40kDa.
Trusted by the scientific community
Anti-NeuroD1 [3H8] - BSA and Azide free (ab60704) was first used in a scientific publication in 2007 and has been cited over 50 times in peer-reviewed journals.
Reviewed by scientists
Anti-NeuroD1 [3H8] - BSA and Azide free (ab60704) has over 15 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NeuroD1 also referred to as Neurogenic Differentiation 1 is a basic helix-loop-helix transcription factor with a molecular mass of approximately 40 kDa. This protein is expressed mainly in the central nervous system as well as in the pancreas. NeuroD1 plays a role in activating transcription by binding to DNA sequences in specific target genes. This process involves dimerization with E proteins facilitating its function as a transcriptional activator.
Biological function summary
NeuroD1 influences neural development and differentiation. It is vital in converting undifferentiated neural precursors into mature neurons ensuring proper brain formation. NeuroD1 forms part of a transcriptional complex with other neural transcription factors which coordinates the expression of genes needed for neuron specification. Apart from neurogenesis it regulates insulin gene expression in pancreatic beta cells linking neural functions to endocrine regulation.
Pathways
NeuroD1 functions in the neurogenesis pathway and interacts closely with the Notch signaling pathway. It plays a role by influencing the expression of genes essential for neuronal lineage commitment. In the insulin signaling pathway NeuroD1 cooperates with other transcription factors like Pdx1 and MafA managing the expression of insulin and other important beta-cell genes. Interactions with proteins like E47 increase its regulatory effects on these pathways.
Defects in NeuroD1 function connect to conditions such as diabetes mellitus and medulloblastoma. In diabetes mellitus NeuroD1 mutations can disturb insulin gene regulation impacting glucose metabolism severely. The connection with Pdx1 further magnifies its involvement in pancreatic beta-cell function. In medulloblastoma aberrant expression or mutations in NeuroD1 affect neuronal differentiation leading to tumor development. NeuroD1 influences cellular pathways interacting with proteins such as Sox9 which contributes to oncogenic processes in neural tissues.