Abcam, ab6148, Anti-IRAK2 antibody

Size: 100µg
Rabbit Polyclonal IRAK2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human IRAK2 aa 500-600.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human IRAK2 aa 500-600. The exact immunogen used to generate this antibody is proprietary information.O43187,
Specificity:Anti-IRAK2 has no cross response to IRAK.

Product details:
The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-Purified IgG was prepared by affinity chromatography., Storage buffer-pH: 7.2Preservative: 0.02% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
IRAK2 also known as Interleukin-1 Receptor-Associated Kinase 2 plays a central role in the immune response. This protein has a molecular mass of approximately 68 kDa. It is broadly expressed in various tissues particularly in those related to the immune system including the spleen thymus and peripheral blood leukocytes. IRAK2 is a part of the IRAK family which includes other kinases like IRAK1 IRAK3 and IRAK4 sharing structural similarities.
Biological function summary
The IRAK2 protein is critical for transducing signals from interleukin-1 receptors (IL-1R) and Toll-like receptors (TLR) as part of the innate immune response. It does not work alone but as part of the Myddosome complex. This complex formation is essential for signal propagation leading to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and mitogen-activated protein kinases (MAPKs). Activation of these pathways initiates inflammatory and immune processes enabling the body to respond to pathogens.
Pathways
IRAK2 integrates into the Toll-like receptor signaling pathway acting as a link between upstream receptor activation and downstream transcription factors. It has strong interactions with other key immune components like MyD88 and TRAF6 within these pathways. Through these links IRAK2 contributes to the orchestration of a coordinated immune defense facilitating the body's capacity to manage microbial infections while maintaining regulatory checks to prevent excessive inflammation.
Defects or dysregulation in IRAK2 function can lead to immune-related diseases. For instance aberrant IRAK2 activity associates with rheumatoid arthritis where it drives inflammatory processes contributing to joint damage. Moreover its involvement in the immune signaling network makes it relevant in sepsis a condition resulting from an overwhelming immune response to infection. In these disorders IRAK2 works in conjunction with other proteins such as IRAK1 and TRAF6 influencing disease progression and symptom manifestation.