Abcam, ab6166, Anti-TRPV1 antibody

Size: 100µL
Anti-TRPV1 antibody (ab6166) is a rabbit polyclonal antibody detecting TRPV1 in Western Blot, IHC-Fr . Suitable for Mouse . - Over 20 publications - Trusted since 2000
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:IHC-FrFl, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Rat Trpv1 aa 800 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.O35433

Product details:
What is this antibody validated in?
Anti-TRPV1 antibody (ab6166) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-Fr) in Mouse samples.
What is the molecular weight of TRPV1?
Anti-TRPV1 (ab6166) specifically detects a band for TRPV1 (UniProt: Q8NER1) at a molecular weight of 94.976kDa.
Trusted by the scientific community
Anti-TRPV1 (ab6166) was first used in a scientific publication in 2000 and has been cited over 20 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Add glycerol to a final volume of 50% for extra stability and aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRPV1 also known as the transient receptor potential vanilloid 1 or capsaicin receptor is a non-selective cation channel with a molecular mass of approximately 95 kDa. It acts mechanically by allowing the influx of calcium and sodium ions upon activation. This receptor is expressed predominantly in sensory neurons specifically in dorsal root ganglia and trigeminal ganglia which are key regions involved in pain perception. TRPV1 can also be found in various non-neuronal tissues influencing a range of physiological responses.
Biological function summary
The role of TRPV1 extends beyond sensory perception. It is involved in detecting and regulating body temperature and is activated by heat acidic conditions and certain lipid metabolites. The receptor operates as part of a homotetrameric complex contributing to neural signaling. It is activated by chemical ligands such as capsaicin the compound responsible for the spicy sensation in chili peppers influencing pain and inflammation pathways.
Pathways
TRPV1 plays essential roles in pain and nociception pathways. It intersects with the inflammatory pathway where its activity is modulated by protein kinase C (PKC) and phospholipase C (PLC). These pathways involve calcium signaling and relate to proteins such as PKA and calcineurin which modulate TRPV1 activity through phosphorylation and dephosphorylation affecting the body's response to noxious stimuli.
TRPV1 is a critical player in pain management and inflammatory disorders. It has been linked to chronic pain conditions like neuropathic pain and is also involved in migraine pathophysiology. In these contexts TRPV1 interacts with proteins like CGRP and TRPA1 which are also implicated in pain signaling and migraine mechanisms. Antagonists such as capsazepine AMG 9810 and AMG-517 have been researched for therapeutic targeting of TRPV1 in these disorders.