Abcam, ab61903, Recombinant human PDGFAA + PDGFBB protein

Size: 10µg / 100µg
Recombinant human PDGFAA + PDGFBB protein is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Escherichia coli,
Tags:Tag free,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Biological Activity: The activity is determined by the dose-dependent proliferation of mouse 3T3 indicator cells and is typically less than 1 ng/mL.,
Accession:P04085,
Animal free:No,
Carrier free:No,
Species:Human,
Reconstitution:Reconstitute at 0.1 mg/mL in water,
Storage buffer:Constituents: 0.06% Acetic acid

Product details:
It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The platelet-derived growth factors PDGFAA and PDGFBB are key proteins that function as homodimers or heterodimers. PDGFAA has a molecular weight of approximately 28 kDa and PDGFBB weighs about 24 kDa. Both proteins localize to various tissues including those involved in wound healing like the skin muscle and blood vessels. They bind to their respective receptor tyrosine kinases PDGF receptors α and β and initiate signaling cascades essential for cellular proliferation and differentiation.
Biological function summary
PDGFAA and PDGFBB play significant roles in cellular processes such as growth survival and motility. These proteins often operate as part of a receptor-ligand complex that mediates signal transduction across the cell membrane. This regulatory action is vital for proper development and repair of connective tissues. The interactions between PDGFAA PDGFBB and their receptors contribute to angiogenesis and tissue remodeling highlighting their importance in both normal physiology and pathological conditions.
Pathways
PDGFAA and PDGFBB are integral to the mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) pathways. These pathways are critical for transmitting extracellular signals into the cell promoting cellular survival and division. The PDGFAA and PDGFBB-driven pathways interact with related proteins like Ras and Akt effectively managing cellular responses to external stimuli. Their involvement in these pathways highlights their importance in regulating the balance between cell growth and apoptosis.
The dysregulation of PDGFAA and PDGFBB links to pathological conditions such as atherosclerosis and cancer. In atherosclerosis PDGFBB collaborates with other growth factors like VEGF to incite smooth muscle cell proliferation and migration contributing to plaque formation. In certain types of cancers PDGFAA and PDGFBB overexpression supports tumor growth and metastasis involving protein partners within the tumor microenvironment. Understanding these pathways may help target therapies in these and related disorders.