Abcam, ab6214, Anti-Orexin A antibody

Size: 100µL
Anti-Orexin A antibody (ab6214) is a rabbit polyclonal antibody detecting Orexin A in IHC-P, IHC-Fr, ELISA . Suitable for Cow, Mouse, Rat . - Over 10 publications - Trusted since 2000
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Mouse, Cow,
Applications:ELISA, IHC-FoFr, IHC-Fr, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Cow HCRT aa 1 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P56717,
Specificity:Immunohistochemical analysis of rat brain indicates a good specificity for this serum.

Product details:
What is this antibody validated in?
Anti-Orexin A antibody (ab6214) is a rabbit polyclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), ELISA in Cow, Mouse, Rat samples.
Trusted by the scientific community
Anti-Orexin A (ab6214) was first used in a scientific publication in 2000 and has been cited over 10 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Storage buffer-pH: 7 - 7.4, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Add glycerol to a final volume of 50% for extra stability and aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Orexin A also known as orexin-a peptide or orexin a is a neuropeptide involved in various physiological processes. It has a molecular mass of approximately 3.5 kDa and consists of 33 amino acids. Orexin A is mainly produced in the lateral hypothalamus. It has a significant presence in the central nervous system where it binds to orexin receptors to stimulate neuronal activity. Despite being small it plays an important role in modulating multiple physiological functions across different systems in the body.
Biological function summary
Orexin A influences sleep-wake cycles and energy homeostasis. It acts independently not as part of a larger protein complex. It promotes wakefulness and regulates feeding behavior by activating orexin receptors which are G-protein-coupled receptors. These receptors are widely distributed throughout the brain indicating orexin A's role in maintaining alertness and energy expenditure. Furthermore it has interactions with several neuropeptides and neurotransmitters affecting reward systems and stress responses.
Pathways
The involvement of orexin A spans the sleep-wake regulation and energy metabolism pathways. This neuropeptide's function in the sleep-wake cycle involves interaction with neurotransmitters like norepinephrine and dopamine which enhance arousal. In energy metabolism it collaborates with proteins such as leptin and ghrelin influencing appetite and feeding behavior. Orexin A's ability to activate these pathways is important for the maintenance of energy balance and vigilance.
Orexin A has strong connections to narcolepsy and metabolic disorders such as obesity. In narcolepsy a deficiency or lack of orexin neurons leads to disrupted sleep-wake cycles resulting in excessive daytime sleepiness. Orexin antagonist drugs have been studied for their potential in treating insomnia by modulating its pathway. In metabolic disorders orexin A’s regulatory function on appetite links it to obesity where an imbalance in orexin signaling might affect energy expenditure. It interacts with leptin and insulin indicating its pivotal role in maintaining metabolic homeostasis.