Abcam, ab62229, Native human Factor Xa

Size: 100µg
Native human Factor Xa is a Human Full Length protein with >95% purity and suitable for SDS-PAGE and Functional studies. The predicted molecular weight of ab62229 native protein is 46 kDa. - Save time and ensure accurate results - use our Factor Xa protein as a control - Optimal protein bioactivity and stability
Key facts
Purity:>95% SDS-PAGE,
Expression system:Native,
Tags:Tag free,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific Activity: Determined using a clotting assay where one unit of activity is equivalent to the factor X activity in one milliliter of normal plasma. Min activity = 700 U/mg; Max activity = 1300 U/mg.,
Accession:P00742,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:Constituents: 50% Glycerol (glycerin, glycerine)

Product details:
The Native Human Factor Xa ab62229 is prepared from plasma shown to be non reactive for anti-HIV-1/2, HIV-1 antigen(s), HBsAg, STS, anti-HCV, anti-HBcore and anti-HTLV I & II.
The bioactivity of Native Human Factor Xa ab62229 was determined by Factor X clotting assay. One (1) unit is equivalent to the Factor X activity in one (1) milliliter of normal human plasma.
Check out our protein gel staining guide for SDS-PAGE
Protein Determination: Extinction Coefficient (ε) 1% at 280 nm, 1cm pathway = 11.6

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Factor Xa also known as activated factor X is an important serine protease in the coagulation cascade. With a molecular mass around 37 kDa this enzyme is an important component in blood coagulation. Factor Xa derives from the hepatic synthesis of factor X requiring vitamin K for its gamma-carboxylation and it is found circulating within the blood plasma. The conversion of factor X into factor Xa occurs when exposed to either extrinsic or intrinsic coagulation pathways.
Biological function summary
Activated factor X plays a pivotal role in converting prothrombin into thrombin facilitating the cross-linking of fibrin which stabilizes the forming clot. Factor Xa while acting as a monomer often associates with factor Va to form prothrombinase complex enhancing its efficiency. The prothrombinase complex accelerates thrombin generation exponentially which is an essential step in hemostasis.
Pathways
The blood coagulation cascade involves factor Xa prominently within the intrinsic and extrinsic pathways. Factor Xa lies downstream in these pathways and links them to the common pathway. It directly interacts with thrombin and factor Va forming an integral part of both pathways in the conversion of prothrombin to thrombin. These steps are important in maintaining hemostasis and enabling proper clot formation during vascular injury.
Factor Xa is highly relevant in coagulopathies such as deep vein thrombosis (DVT) and pulmonary embolism (PE). Aberrant activity of this factor contributes to excessive clot formation leading to thrombotic disorders. Additionally factor Xa is a target for anticoagulant therapies such as rivaroxaban and apixaban which inhibit its activity to prevent thrombotic events. Such inhibitors affect conditions related to factor Xa impacting the risk and management of cardiovascular diseases.