Abcam, ab62321, Anti-Insulin Receptor (phospho Y1185) antibody [EP351(2)Y]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal Insulin Receptor phospho Y1185 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 8 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP351(2)Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
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contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The insulin receptor (INSR) is a transmembrane glycoprotein that functions as a receptor for insulin. Known also as CD220 the receptor has a molecular weight of about 320 kDa. It is widely expressed in insulin-sensitive tissues such as liver muscle and adipose tissues. The insulin receptor consists of two α subunits and two β subunits forming a heterotetramer. These subunits are located outside and inside the cell membrane respectively. The α subunits bind insulin while the β subunits possess tyrosine kinase activity important for downstream signaling.
Biological function summary
Insulin receptor plays a role in facilitating the effects of insulin on glucose and lipid metabolism. When insulin a protein binds to the insulin receptor it undergoes a conformational change that activates its intrinsic kinase activity. This activation further leads to tyrosine phosphorylation of intracellular targets resulting in modulation of cellular functions. Insulin receptor also participates in the formation of signaling complexes through interaction with substrates like insulin receptor substrate (IRS) proteins which are important for the transmission of the insulin signal inside cells.
Pathways
Insulin receptor is central to the insulin signaling pathway and the mitogen-activated protein kinase (MAPK) pathway. Activation of the insulin receptor triggers the insulin signaling cascade which involves various proteins like PI3 kinase and Akt that contribute to glucose uptake and metabolism. In the MAPK pathway the insulin receptor influences gene expression related to cell growth and differentiation. These pathways intertwine with other hormone signaling systems and affect numerous physiological processes critical for maintaining metabolic homeostasis.
Aberrations in insulin receptor function are linked to insulin resistance and type 2 diabetes. Defective signaling through this receptor impairs glucose transport into cells contributing to hyperglycemia. Disorders in insulin receptor activity also relate to metabolic syndrome a cluster of conditions increasing the risk of heart disease. Proteins connected to these conditions include IRS proteins and PI3 kinase highlighting the integrated roles these components play in metabolic regulation. Developing anti-insulin receptor antibodies offers potential therapeutic interventions to modulate receptor function and manage these disorders effectively.