Abcam, ab62721, Anti-LC3A/B antibody

Size: 50µL
Rabbit Polyclonal LC3B antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples. Cited in 65 publications. Immunogen corresponding to Synthetic Peptide within Human MAP1LC3A aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human, Mouse,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MAP1LC3A aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.Q9H492

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The target proteins LC3A and LC3B also known as MAP1LC3A and MAP1LC3B are part of the microtubule-associated protein 1 light chain 3 family. Each of these proteins has a molecular mass of approximately 17 kDa. They are present in various tissues throughout the body including the brain liver and heart. LC3A/B proteins are expressed in both the cytoplasm and autophagic vesicles where they play important roles in autophagy mechanisms.
Biological function summary
LC3A/B proteins serve as central markers and mediators in the autophagy process where they are involved in the formation of autophagosomes. These proteins exist as part of the complex machinery that includes other autophagy proteins like ATG7 and ATG3. LC3A/B facilitate the elongation and closure of the phagophore membrane forming complete autophagosomes that encapsulate cellular debris and damaged organelles for degradation.
Pathways
LC3A/B proteins are integral to the autophagy pathway which maintains cellular homeostasis by recycling cellular components. Autophagy is linked to the mTOR signaling pathway where mTOR acts as an important regulator. LC3A/B interacts with the ubiquitin-proteasome system and proteins like p62/SQSTM1 that recognize ubiquitinated cargo directing them toward degradation in autophagosomes.
LC3A/B proteins are implicated in conditions like neurodegenerative diseases including Parkinson's disease where autophagy dysfunction plays an important role. Aberrant regulation of LC3A/B and connected proteins like alpha-synuclein contribute to the pathology of these diseases. LC3A/B is also associated with cancer where alterations in autophagic activity often lead to tumor progression or suppression depending on the context and tumor type.