Abcam, ab62734, Anti-Nanog antibody [NNG-811]

Size: 50µL
Anti-Nanog antibody [NNG-811] (ab62734) is a mouse monoclonal antibody detecting Nanog in Western Blot, IP, ICC/IF . Suitable for Human . - Over 40 publications - Trusted since 2008
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:NNG-811,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human NANOG.Q9H9S0

Product details:
What is this antibody validated in?
Anti-Nanog antibody [NNG-811] (ab62734) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of Nanog?
Anti-Nanog [NNG-811] (ab62734) specifically detects a band for Nanog (UniProt: Q9H9S0) at a molecular weight of 35kDa.
Trusted by the scientific community
Anti-Nanog [NNG-811] (ab62734) was first used in a scientific publication in 2008 and has been cited over 40 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Purification notes-Purified Immunoglobulin, Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Nanog also known as Nanog homeobox is a transcription factor playing an important role in maintaining the pluripotency of embryonic stem cells. The molecular weight of Nanog is approximately 35 kDa. It is found mainly in the inner cell mass of the blastocyst but it also expresses in embryonic stem cells and some adult stem cell populations. Nanog acts by binding to DNA in a sequence-specific manner to regulate gene expression essential for stem cell self-renewal and pluripotency.
Biological function summary
Nanog plays a central role in stem cell biology operating as part of a complex regulatory network. Nanog interacts with other important transcription factors like Oct4 and Sox2 forming a core pluripotency network. This network suppresses the differentiation of stem cells by regulating the expression of genes involved in cell fate decisions. By modulating the expression of different pathways Nanog ensures the maintenance of an undifferentiated state in stem cells.
Pathways
Nanog is deeply embedded in the Wnt/Β-Catenin and TGF-Β signaling pathways important for stem cell maintenance and differentiation. In the Wnt/Β-Catenin pathway Nanog works alongside proteins like Β-catenin to drive the expression of genes that promote self-renewal. Meanwhile in the TGF-Β pathway Nanog acts with proteins such as Smad2/3 to balance pluripotency and differentiation signals. These pathways critically support the complex network that sustains stem cell identity and function.
Nanog's expression relates closely to its role in various cancers such as glioblastoma and colorectal cancer. Abnormally high levels of Nanog contribute to the tumorigenicity of cancer cells by maintaining their self-renewal and undifferentiated state similar to its role in stem cells. Nanog interacts with proteins like p53 known for its tumor suppressor functions often leading to challenges in cancer treatment. Understanding Nanog's influence in these pathways provides valuable insights into therapeutic targets for combating cancer stem cell resilience.