Abcam, ab6308, Anti-Collagen I antibody [COL-1]

Size: 50µL
Anti-Collagen I antibody [COL-1] (ab6308) is a mouse monoclonal antibody detecting Collagen I in Western Blot, IHC-Fr . Suitable for Human . - Over 460 publications - Trusted since 2000
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:COL-1,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:IHC-Fr, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Cow COL1A1.P02453,
Epitope:The epitope recognized by the antibody may be sensitive to routine formalin fixation and paraffin embedding. There have been varying results when using this antibody in IHC-P. Please refer to our customer Abreviews for more protocol information and optimization steps when using this antibody in IHC-P.

Product details:
Anti-Collagen I antibody [COL-1] (ab6308) is a House Mouse Monoclonal antibody and is validated for use in IHC-Fr, WB.
Anti-Collagen I antibody [COL-1] (ab6308) has been cited over 467 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality validation processes ensure Anti-Collagen I antibody [COL-1] (ab6308) has high sensitivity and specificity.
Anti-Collagen I antibody [COL-1] (ab6308) has 33 independent reviews from customers.
Anti-Collagen I antibody [COL-1] (ab6308) specifically detects Collagen I (UniProt ID: P02452; Molecular weight: 95kDa) and is sold in 50 uL selling sizes.
Collagen I (COL1A1)is a major component of the extracellular matrix (ECM) and plays a significant role in cancer progression. In the tumor microenvironment, Collagen I can promote tumor growth, invasion, and metastasis by increasing tissue stiffness and facilitating cancer cell migration.
Production of this antibody has been changed on 23
June 2016. The following lots are from ascites and are still in stock as of 23
June 2016 : GR210978, GR175242, GR158374. Lot numbers higher than GR210978 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

Properties and Storage Information:
Form-Liquid, Purification technique-Proprietary technique, Purification notes-Purified from Tissue culture supernatant., Storage buffer-pH: 7.4Preservative: 0.0976% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.
Biological function summary
Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.
Pathways
Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.
Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.