Abcam, ab63553, Anti-NEK9 (phospho T210) antibody

Size: 100µg
Rabbit Polyclonal NEK9 phospho T210 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NEK9 phospho T210.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human NEK9 phospho T210. The exact immunogen used to generate this antibody is proprietary information.Q8TD19

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NEK9 also known as NIMA-related kinase 9 is a serine/threonine kinase with a molecular mass of approximately 118 kDa. It plays a role in the regulation of various cell cycle processes. NEK9 is active in both the cytoplasm and nucleus and expresses broadly in various tissues including the liver kidneys and pancreas. It also has homologs in other eukaryotic organisms indicating its conserved function across species.
Biological function summary
This protein modulates different aspects of mitosis particularly in spindle formation and chromosome segregation. NEK9 forms a part of a complex in conjunction with other kinases like NEK6 and NEK7 coordinating the early events of mitosis. Its activity supports centrosome separation and microtubule nucleation which are important for proper cell division.
Pathways
NEK9 is involved in the cell cycle and microtubule organization pathways. It works in conjunction with proteins such as PLK1 and Aurora A which regulate the entry and progression through mitosis. NEK9 activates NEK6 and NEK7 which further propagate signals necessary for mitotic spindle assembly ensuring efficient cell division.
NEK9 has links to cancer and neurological disorders. Abnormal NEK9 activity has associations with tumorigenesis particularly linked with the dysregulation of cell cycle checkpoints and malignant transformation. In neurological disorder contexts NEK9 interacts with proteins like tau perturbing normal cellular functions and potentially contributing to disease pathology.