Abcam, ab63827, Recombinant E. coli RuvB protein (Active)

Size: 20µg
Recombinant E. coli RuvB protein (Active) is a Escherichia coli K-12 Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, ELISA, WB, FuncS.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:Tag free,
Applications:FuncS, WB, SDS-PAGE, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P0A812,
Animal free:No,
Carrier free:No,
Species:Escherichia coli K-12,
Storage buffer:pH: 6 - 8.5Constituents: 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.158% Tris HCl, 0.0584% EDTA, 0.039% 2-Mercaptoethanol

Product details:
This protein can be used for:- 1) Studies on homologous recombination mechanism. 2) To make use of the motor protein function that specifically migrates the Holliday junction by forming a complex with RuvA (branch-migration protein).

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RuvB also known as RuvB-like 1 and 2 (RUVBL1/RUVBL2) is a highly conserved ATP-dependent helicase with a molecular mass of roughly 51 kDa. This protein can unwind DNA a function that plays an essential role in DNA repair and recombination. It is expressed in various tissues across different species with high expression in rapidly dividing cells and tissues undergoing repair. RuvB forms hexameric or dodecameric rings which allow it to interact with other protein complexes and nucleic acids.
Biological function summary
The RuvB proteins participate in the regulation of essential cellular events. They integrate into larger multi-protein complexes such as the chromatin remodeling complex INO80 and the TIP60 complex which are involved in modulating nucleosome structure. In the context of chromatin remodeling RuvB helps regulate access to the DNA by other proteins and enzymes affecting transcription DNA repair and replication. These activities underline RuvB's role in maintaining genomic stability.
Pathways
The participation of RuvB in key regulatory pathways includes DNA damage response and transcription regulation. In the DNA damage repair pathway it collaborates with proteins like RAD51 and BRCA1 to resolve DNA breaks. Additionally in the transcriptional regulation pathway RuvB interacts with RNA Polymerase complexes and is implicated in the regulation of gene expression. These interactions illustrate how RuvB is important for cellular responses to various stimuli that require rapid changes in gene transcription profiles and maintenance of DNA integrity.
Mutations or dysregulation of RuvB can contribute to the development of cancer and neurological disorders. Cancer often involves RuvB in its chromatin remodeling role where altered RuvB function can lead to unchecked cell proliferation due to improper DNA repair and transcription. Furthermore in some neurological disorders abnormal interaction between RuvB and proteins like Huntingtin has been observed suggesting a link between RuvB function and the pathogenic mechanisms in Huntington’s disease. These associations highlight RuvB as a significant target for therapeutic research in oncology and neurobiology.