Abcam, ab64257, Biotinylated Goat anti-Mouse & Rabbit IgG (H+L) (Ready To Use)

Size: 125mL
Suitable for IHC-P. Cited in 10 publications.
Key facts
Host species:Goat,
Target species:Rabbit,
Target isotype:IgG,
Target specificity:Heavy & Light chains,
Pre-adsorbed:No,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Clonality:Polyclonal

Product details:
Staining protocol
Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
Add enough drops of an appropriate hydrogen peroxide block to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer.
Perform appropriate pretreatment if required. Wash 3 times in buffer.
Apply an appropriate protein block and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash 1 time in buffer.
Apply primary antibody and incubate according to manufacturer's protocol.
Wash 4 times in buffer. Apply Biotinylated Goat anti Mouse & Rabbit IgG(H+L) solution (ab64257) and incubate for 10 minutes at room temperature. Wash 4 times in buffer.
Apply enzyme labeled streptavidin and incubate according to manufacturer's protocol.
Rinse 4 times in buffer. Place slide in appropriate chromogenic substrate and incubate until desired reaction is achieved. Rinse 4 times in buffer.
Add enough drops of Hematoxylin to cover the section. Incubate for 1 minute.
Rinse 7-8 times in tap water. Add Mounting Medium to cover the section.
This reagent is intended to be used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.2 - 7.6Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C