Abcam, ab64269, Streptavidin HRP (ready-to-use)

Size: 125mL
Streptavidin HRP (ready-to-use) (ab64269) reagent is used to detect biotinylated antibodies in immunohistochemistry (IHC) assays. - Proven performance: cited in over 60 publications - Forms a strong, specific non-covalent complex with biotin - Has less non-specific binding and less background signal compared to avidin - Compatible with our Lab essentials imaging workflow offering
Key facts
Tags:Tag free,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P22629,
Animal free:No,
Carrier free:No,
Species:Streptomyces avidinii,
Storage buffer:pH: 7 - 8Preservative: 0.2% Proclin 300Constituents: PBS, 9% Goat Serum, 1.08% BSA, 0.05% Sorbitan monolaurate, ethoxylated, 0.012% HRP-conjugated streptavidin

Product details:
Streptavidin-HRP for use with biotinylated secondary antibodies during IHC / immunohistochemistry. Optimized, ready-to-use format.
This product was previously called Streptavidin-Peroxidase.
IHC protocol suitable for use with Streptavidin-HRP ab64269:
1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
2. Use appropriate
antigen retrieval buffer or enzyme
(primary antibody dependent) to treat sections. Wash 3 times in buffer.
3. Add enough
hydrogen peroxide blocking solution
to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, use
avidin biotin blocking
4. Apply
protein block
(or normal serum from same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.
5. Apply primary antibody in
antibody diluent
and incubate.
6. Wash 4 times in buffer. Incubate slide with
biotinylated secondary antibody
HRP polymer secondary antibody
and skip step 7). Wash 4 times in buffer.
Apply streptavidin-HRP ab64269 and incubate for 10 minutes at room temperature.
8. Rinse 4 times in buffer. Place slide in
DAB substrate
AEC substrate
and incubate until desired color is achieved. Rinse 4 times in buffer.
9. Add enough drops of
hematoxylin
to cover the section. Incubate for 1 minute.
10. Rinse 7-8 times in tap water. Add
mounting medium
to cover the section.
Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the
IHC kits and reagents guide

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C