Abcam, ab64845, Anti-DGKA antibody

Size: 100µg
Rabbit Polyclonal DGKA antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human DGKA aa 300-400.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human DGKA aa 300-400. The exact immunogen used to generate this antibody is proprietary information.P23743

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DGKA also known as diacylglycerol kinase alpha plays an important role in the conversion of diacylglycerol (DAG) to phosphatidic acid (PA) by transferring a phosphate group from ATP. This enzymatic process regulates levels of DAG and PA which are critical molecules for signaling processes. DGKA has a molecular weight of approximately 80 kDa. Expression of DGKA occurs in various tissues with notable presence in the brain and immune system highlighting its significance in neurological and immunological functions.
Biological function summary
DGKA modulates multiple signaling pathways by controlling the availability of DAG and PA essential intermediates in intracellular signaling. It operates as part of a complex that interacts with various phosphorylated proteins to affect downstream signaling. The enzyme influences cellular processes such as proliferation differentiation and apoptosis by modulating DAG-dependent signaling pathways.
Pathways
DGKA integrates into significant signaling cascades including the PI3K/Akt and MAPK pathways. In the PI3K/Akt pathway DGKA modulates the activity of proteins like PKC through its DAG-regulating function. Alternatively in the MAPK pathway it influences protein phosphorylation through effects on upstream kinases. This regulation highlights the enzyme's role in critical cell fate decisions.
DGKA has associations with certain cancers and immune-related diseases. Aberrant DGKA activity links to oncogenic processes influencing tumor growth and progression via its impact on the PI3K/Akt pathway. In immune disorders dysregulated DGKA activity connects to excessive inflammation affecting proteins such as PKC that are involved in immune responses. Understanding DGKA's role in these diseases could drive new therapeutic strategies.