Abcam, ab6650, FITC Anti-Biotin antibody

Size: 100µg
Goat Polyclonal Biotin antibody - conjugated to FITC. Suitable for Flow Cyt, ICC/IF, Dot and reacts with Chemical samples. Cited in 6 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Biotin.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Conjugation:FITC,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Applications:Dot, Flow Cyt, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.7 moles FITC per mole of Goat IgG

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads., Storage buffer-Preservative: 0.01% Sodium azideConstituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Biotin also known as vitamin B7 or vitamin H is a water-soluble B vitamin with a molecular weight of 244.31 g/mol. It mechanically functions as a coenzyme for carboxylase enzymes which are critical in facilitating carboxylation reactions where carbon dioxide is added to a substrate. These reactions are significant in the metabolism of fatty acids amino acids and carbohydrates. Biotin is widely expressed in various tissues including the liver kidney and brain making it essential for normal cellular functions.
Biological function summary
Biotin acts as a fundamental component in energy production and nutrient metabolism. It forms a covalent bond with specific carboxylase enzymes like acetyl-CoA carboxylase and pyruvate carboxylase forming a protein-biotin complex. This binding allows these enzymes to perform their carboxylation reactions effectively impacting multiple metabolic pathways. Biotin also participates in gene regulation and cellular signaling processes which are essential for normal growth and development of cells.
Pathways
Biotin plays a role in fatty acid synthesis and gluconeogenesis pathways. It aids in the conversion of substrates into products necessary for storing and producing energy. For example in the fatty acid synthesis pathway biotin-dependent acetyl-CoA carboxylase catalyzes the conversion of acetyl-CoA to malonyl-CoA an important step in synthesizing fatty acids. Biotin works alongside other proteins such as holocarboxylase synthetase to ensure proper enzyme function in these pathways reflecting its interconnected role in metabolic processes.
Biotin deficiency can lead to conditions such as biotinidase deficiency and multiple carboxylase deficiency. These disorders impair the body's ability to recycle or utilize biotin efficiently leading to metabolic issues. Biotin deficiency is also associated with skin rashes hair loss and neurological symptoms as biotin is connected to proteins like biotinidase and holocarboxylase synthetase which play roles in enzyme function and biotin recycling. Proper biotin levels are essential for maintaining metabolic balance and preventing such disorders.