Abcam, ab66978, Anti-HSP1 / Protamine P1 antibody

Size: 50µg
Mouse Polyclonal HSP1 / Protamine P1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PRM1.
Key facts
Host species:Mouse,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human PRM1.P04553

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HSP1 / Protamine P1 also known as PRM1 is a small arginine-rich nuclear protein. It has a mass of approximately 6.3 kDa. Protamine P1 primarily expresses in the testis especially during spermatogenesis. It plays an important mechanical role in replacing histones during sperm chromatin remodeling facilitating the condensation of sperm DNA into an exceptionally compact and stable structure. This condensation is essential for protecting genetic material during transport.
Biological function summary
The protein serves a fundamental role in ensuring male fertility through its contribution to chromatin packaging. Protamine P1 does not function independently; instead it forms a complex with other protamines such as Protamine P2 (PRM2) to adequately condense the DNA in spermatids. The tight binding of Protamine P1 to DNA minimizes transcription during the final stages of sperm development preserving the genetic integrity of future offspring.
Pathways
Protamine P1's activity integrates mainly into the sperm development pathway. During this pathway it interacts closely with proteins involved in chromatin assembly and disassembly. H1 a linker histone is one such protein that transitions out of the chromatin to allow Protamine P1 to facilitate nucleosome elimination. This pathway ensures that DNA compaction and protection are achieved setting the stage for successful fertilization.
Protamine P1's role is critical in understanding male infertility and certain cases of protamine-deficiency. Abnormal expression levels or structural changes in Protamine P1 correlate with male infertility due to improper chromatin packaging. These alterations often associate with genetic problems in Protamine P2 or disruptions in the balance between the two protamines. Furthermore studies have linked Protamine P1 to increased DNA fragmentation in sperm which can contribute to unsuccessful zygote formation.