Abcam, ab67800, Anti-TRFP/MED20 antibody

Size: 50µg
Mouse Polyclonal TRFP/MED20 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MED20.
Key facts
Host species:Mouse,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human MED20.Q9H944

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRFP also known as MED20 is part of the Mediator complex which acts as a communication bridge between gene-specific regulatory proteins and RNA polymerase II. MED20 has a molecular mass of approximately 22 kDa and is essential for the transcriptional coactivation process. This protein is highly expressed in various human tissues including the liver heart and kidneys with detectable levels in several other organs. MED20 facilitates the activation of transcription by interacting with multiple transcription factors.
Biological function summary
The Mediator complex which includes MED20 as one of its subunits plays a significant role in the initiation of transcription. MED20 forms part of the larger Mediator complex working closely with other subunits to modulate gene expression. By serving as a scaffold it helps stabilize interactions between transcription factors and RNA polymerase II enhancing transcription efficiency. The complex influences cell growth differentiation and response to cellular signals.
Pathways
MED20's presence in the Mediator complex situates it in critical transcription regulation pathways such as the RNA polymerase II transcription initiation pathway. It interacts with proteins like CDK8 and CCNC integral in controlling transcription by regulating Mediator's cyclin-dependent kinase activity. MED20 fosters connections between regulatory proteins and RNA polymerase II affecting transcription-related cellular signaling.
Abnormalities in the Mediator complex including issues with MED20 have implications in certain disorders like cancer and cardiovascular disease. Dysregulation of gene expression through interrupted Mediator activity can lead to these conditions. Specifically deficiencies involving MED20 affect the overall integrity of the Mediator complex relating to proteins such as MED12 and MED1 which have associations with oncogenesis and developmental defects.