Abcam, ab68023, Anti-AFG3L2 antibody

Size: 50µg
Mouse Polyclonal AFG3L2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human AFG3-like protein 2.
Key facts
Host species:Mouse,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human AFG3-like protein 2.Q9Y4W6

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AFG3L2 also known as AFGL2 is a mitochondrial protein with a mass of approximately 90 kDa. It belongs to the AAA protease family and functions as a metalloprotease. You can find AFG3L2 expressed mainly in the brain heart and skeletal muscles. It resides in the inner mitochondrial membrane where it plays a mechanical role in protein quality control and processing eliminating misfolded or damaged proteins within the mitochondria.
Biological function summary
AFG3L2 is important for maintaining mitochondrial integrity and functional protein homeostasis. AFG3L2 forms a part of the m-AAA protease complex along with its homolog SPG7. This complex is integral for the regulation of mitochondrial ribosome assembly and is involved in regulating the mitochondrial respiratory chain complexes. AFG3L2’s role in processing several protein precursors helps ensure efficient mitochondrial function highlighting its importance in maintaining cellular energy balance.
Pathways
AFG3L2’s involvement spans across critical mitochondrial processes including the OXPHOS (oxidative phosphorylation) pathway and mitochondrial biogenesis. The activity of AFG3L2 affects key proteins like OPA1 which highlights its role in mitochondrial dynamics and energy metabolism. Moreover this protein’s interactions also influence the activity and stability of diverse respiratory chain complexes establishing it as an important player in cellular energy production.
AFG3L2 mutations have been linked to spinocerebellar ataxia type 28 (SCA28) and optic atrophy highlighting its relevance to neurodegenerative conditions. These conditions result from disruptions in mitochondrial function emphasizing the importance of AFG3L2 in neuronal health. It also connects with proteins such as paraplegin with which it exerts synergistic effects and abnormalities in this interaction often worsen the expression and impact of both SCA28 and related pathologies.