Abcam, ab68164, Anti-PDK2 antibody [EPR1948Y]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal PDK2 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Rat, Mouse samples. Cited in 30 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR1948Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IP, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PDK2 also known as Pyruvate Dehydrogenase Kinase 2 is a mitochondrial protein involved in the regulation of glucose metabolism. It phosphorylates and inactivates the pyruvate dehydrogenase complex (PDC) which plays an important role in controlling the conversion of pyruvate to acetyl-CoA. PDK2 has a molecular mass of approximately 46 kDa and shows expression in various tissues including the heart liver and kidneys. The expression pattern indicates its significance in tissues with high metabolic demands.
Biological function summary
PDK2 regulates cellular energy metabolism by phosphorylating the E1 component of PDC resulting in lowered acetyl-CoA production. PDK2 often acts as part of a regulatory complex with PDC influencing metabolic flux within the mitochondria. Its activity can alter ATP production and fatty acid synthesis impacting overall cellular function and energy homeostasis. In response to different metabolic needs the availability and activity of PDK2 modulate the balance between carbohydrate utilization and lipid oxidation.
Pathways
PDK2 has an important role in the glucose and lipid metabolic pathways by interacting with PDC. The pathways where PDK2 is active are essential for maintaining energy homeostasis and are sensitive to different physiological conditions. PDK2's regulation of pyruvate oxidation influences the tricarboxylic acid (TCA) cycle affecting key metabolic enzymes such as pyruvate dehydrogenase phosphatase 1 (PDP1) and PDK1. Its activity is often fine-tuned by factors impacting energy needs insulin signaling and nutrient availability.
Abnormal PDK2 activity associates with metabolic conditions like diabetes and cancer. In diabetes the dysregulation of PDK2 alters the metabolic processing of carbohydrates leading to increased reliance on lipid oxidation and contributing to hyperglycemia. In cancer PDK2's role in promoting aerobic glycolysis also known as the Warburg effect supports rapid cell growth and survival. The link between PDK2 and these diseases highlights its involvement alongside other proteins such as hypoxia-inducible factor 1-alpha (HIF-1α) in promoting altered energy metabolism in pathological states.