Abcam, ab68536, Anti-SDS antibody

Size: 50µg
Mouse Polyclonal SDS antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SDS.
Key facts
Host species:Mouse,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human SDS.P20132

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Sodium dodecyl sulfate (SDS) also known as sodium lauryl sulfate is an anionic detergent with a molecular weight of 288.38 g/mol. It disrupts non-covalent bonds in proteins causing them to denature. SDS is widely used for its ability to solubilize proteins by coating them in negative charge ensuring uniform migration during electrophoresis. In laboratory settings SDS is most often expressed in solutions prepared for SDS-PAGE a common technique to separate proteins based on molecular weight.
Biological function summary
Scientists employ sodium dodecyl sulfate in the analysis of protein composition and structure. SDS facilitates the study of protein complexes by breaking apart aggregates into individual polypeptide chains. This function is particularly important in research aiming to determine protein characteristics such as size purity and content. Researchers often pair SDS with reducing agents to further break disulfide bonds in proteins offering clearer insights into the proteins' linear arrangement.
Pathways
The use of sodium dodecyl sulfate is important for revealing information underpinned by protein characterization and separation protocols. In processes like the MAPK/ERK signaling pathway understanding individual protein constituents is important. Additionally related proteins such as kinases and phosphatases have their activities scrutinized through SDS-PAGE analysis enabling better assessment of their roles in signaling pathways.
The accuracy of sodium dodecyl sulfate in protein study helps in understanding conditions such as Alzheimer's disease and some cancers. Scientists analyze variations in protein expression and aggregation considering proteins like amyloid-beta and oncogenes in these disorders. SDS makes it possible to detect these alterations early on which helps in advancing diagnostic and therapeutic strategies against such conditions.