Abcam, ab70000, Anti-NANOS2 antibody

Size: 100µg
Rabbit Polyclonal NANOS2 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human NANOS2.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human NANOS2. The exact immunogen used to generate this antibody is proprietary information.P60321,
Specificity:ab70000 does not cross-react with either Nanos1 or Nanos3.

Product details:
Previous batches of ab70000 have been successfully used in IHC-P on testis tissue sections (please see Kusz KM et al., 2009). However, when tested on human brain tissue sections, ab70000 gave negative results in IHC-P (data not shown). We do not guarantee the current batch of ab70000 to work in IHC.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The NANOS2 protein also known simply as NANOS 2 is a member of the NANOS family of proteins and plays an integral role in the regulation of germ cell development. It has a molecular mass of approximately 17 kDa. This protein is primarily expressed in the male germline specifically in spermatogonial stem cells within the testes. Its expression is tightly regulated and is essential for the proper maintenance and differentiation of these stem cells contributing significantly to male fertility.
Biological function summary
NANOS2 acts as a post-transcriptional regulator and it is part of a ribonucleoprotein complex. It helps maintain germ cell pluripotency and influences the cell cycle. Functioning mainly through the binding to the 3' untranslated region (3' UTR) of target mRNAs NANOS2 controls the translation and stability of specific mRNAs that are important for germ cell maintenance and differentiation. This control is essential for preventing premature meiosis or differentiation in male germ cells.
Pathways
The involvement of NANOS2 is critical in both the PI3K/AKT signaling pathway and the TGF-beta signaling pathway. In the PI3K/AKT pathway NANOS2 interacts with proteins such as PI3K and AKT to regulate cell survival and proliferation specifically in germ cells. In the TGF-beta pathway NANOS2 works alongside Smad proteins to modulate responses essential for cell cycle regulation ensuring the proper timing of germ cell differentiation.
Disruptions in NANOS2 expression or function are closely related to infertility particularly affecting spermatogenesis. Studies have shown that NANOS2 abnormalities can lead to male infertility due to inadequate spermatogonial stem cell maintenance and differentiation. Additionally NANOS2 has been linked to testicular germ cell tumors with its expression patterns altered in cancerous cells compared to normal tissue. Such pathological conditions often see interactions with oncogenic proteins like PLZF highlighting the importance of NANOS2 in both normal and disease states of germ cell development.