Abcam, ab70273, Anti-PITX1/BFT antibody

Size: 100µL
Rabbit Polyclonal PITX1/BFT antibody. Suitable for IP, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human PITX1.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PITX1. The exact immunogen used to generate this antibody is proprietary information.P78337

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: PBS, 1.815% Tris, 1.764% Sodium citrate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The PITX1 gene also known as BFT or BFT NZ encodes the Pituitary Homeobox 1 protein. This protein is a homeodomain transcription factor and plays an important role in regulating gene expression by binding to specific DNA sequences. PITX1 has a molecular mass of approximately 33 kDa. It is primarily expressed in tissues like the pituitary gland lower limbs and jaw. The expression in these specific areas hints at its importance in the developmental processes that occur during embryogenesis.
Biological function summary
The PITX1 protein functions as part of the regulation of limb and craniofacial development. It is not part of a larger protein complex but works independently to influence the differentiation and development of specific cell types. In the embryonic stages PITX1 directs the growth and formation of lower limb structures and mutations in this gene can lead to limb malformations. The protein also has roles in craniofacial development highlighting its multifaceted biological functions.
Pathways
PITX1 is involved in the Wnt signaling pathway and the Hedgehog pathway both essential for proper developmental processes. In the Wnt signaling pathway PITX1 interacts with other proteins such as β-catenin to modulate gene expression necessary for limb development. In the Hedgehog pathway PITX1 influences how cells respond to Hedgehog signals affecting cell growth and specialization. The coordinated role of PITX1 in these pathways supports its critical involvement in developmental regulation.
PITX1 has connections to clubfoot and Liebenberg syndrome. Clubfoot a congenital deformity of the foot often links to mutations or alterations in PITX1 expression affecting limb development. Moreover Liebenberg syndrome suggests altered expression or function of PITX1 leading to misregulated limb and joint formation. In both diseases associations with proteins like β-catenin and hedgehog proteins illustrate how changes in PITX1 can result in significant phenotypic abnormalities reflecting its importance in both normal and pathological conditions.