Abcam, ab70300, Anti-REST / NRSF antibody

Size: 100µL
Rabbit Polyclonal REST / NRSF antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab70300 was affinity purified using an epitope specific to REST / NRSF immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: PBS, 1.815% Tris, 1.764% Sodium citrate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
REST also known as NRSF (neuron-restrictive silencer factor) is a transcriptional repressor weighing approximately 116 kDa. It regulates gene expression by binding to the RE1 silencing transcription factor (RE1-silencing element) in the DNA. REST is widely expressed in non-neuronal cells and plays a significant role in maintaining neuronal gene silencing in embryonic and adult stem cells. By interacting with co-repressors REST recruits histone deacetylases and methylases to modify chromatin structure effectively shutting down gene transcription in specific contexts.
Biological function summary
REST regulates neuronal differentiation and plasticity by controlling the expression of neuronal-specific genes. It forms a complex with co-repressors such as mSin3 and CoREST participating in diverse biological processes including neurogenesis. REST expression is important for balancing the neuronal gene expression program preventing untimely or inappropriate activation of neuronal genes in non-neuronal tissues. By modulating the expression of synaptic proteins and neurotransmitter receptors REST influences synaptic plasticity and neuronal excitability.
Pathways
REST participates in the neural stem cell differentiation pathway and cell fate determination. It interacts with pathways involving proteins such as HDAC1 and SWI/SNF complexes contributing to chromatin remodeling and transcriptional regulation. Within these pathways REST ensures the maintenance of non-neuronal identity by repressing neuronal gene activation coordinating with other proteins to influence cellular outcomes and fate decisions.
REST has been linked to neurological disorders such as Huntington's disease and epilepsy. In Huntington's disease REST dissociation from the Huntingtin protein increases leading to misregulation of neuronal genes and contributing to neurodegenerative changes. REST also plays a role in epilepsy where its dysregulation can lead to an imbalance in excitatory and inhibitory signals within the brain potentially triggering epileptic episodes. Through its interactions with disease-associated proteins REST exemplifies the complex interplay between transcriptional regulation and cellular dysfunction in disease pathology.