Abcam, ab70618, Anti-E4F1 antibody

Size: 100µL
Rabbit Polyclonal E4F1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human E4F1 aa 700 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human E4F1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q66K89

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab70618 was affinity purified using an epitope specific to E4F1 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: PBS, 1.815% Tris, 1.764% Sodium citrate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
E4F1 also known as adenovirus E4 promoter-binding protein 1 is a transcriptional regulator with a molecular mass of approximately 120 kDa. This protein is expressed in both the nucleus and cytoplasm of cells. E4F1 plays an essential role in regulating gene expression by modulating transcription factors and interacting with DNA. It is known for its ability to bind to promoters influencing the transcriptional initiation of target genes.
Biological function summary
The role of E4F1 involves cell cycle control apoptosis and DNA repair. E4F1 interacts with a network of proteins forming complexes that are important for its function. These complexes enable E4F1 to influence cellular responses to stress and aid in DNA damage repair mechanisms. E4F1's impact on these biological processes underlines its significance in maintaining cell homeostasis and genomic stability.
Pathways
E4F1 is significantly involved in the p53 signaling pathway and cell cycle control mechanisms. E4F1's role in these pathways is closely connected with the tumor suppressor protein p53 where it modulates p53’s transcriptional activity under stress conditions. Additionally E4F1 interacts with Rb (retinoblastoma protein) in the regulation of cell cycle progression especially during the transition from the G1 to S phase accentuating its contribution to cell cycle checkpoints.
E4F1 has a notable connection with cancer and neurodegenerative conditions. In cancer E4F1’s interaction with p53 influences tumorigenesis as perturbations in this interaction can lead to uncontrolled cell proliferation. In neurodegenerative diseases E4F1’s involvement in DNA repair and apoptosis links it to pathway disruptions that are characteristic of such disorders. E4F1's relationships with proteins like p53 and Rb suggest its potential as both a biomarker and therapeutic target in these diseases.