Abcam, ab70770, Anti-MID1 antibody

Size: 100µg
Rabbit Polyclonal MID1 antibody. Suitable for ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human MID1.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:WB, IHC-P, ELISA, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MID1. The exact immunogen used to generate this antibody is proprietary information.O15344

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab70770 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The MID1 protein also known as midlin or tripartite motif-containing protein 18 plays a significant role in cellular activities. Weighing around 72 kDa this protein consists of numerous domains including the B-boxes and coiled-coil regions. MID1 is usually expressed in the cytoplasm and nucleus of various cell types emphasizing its involvement in cellular processes. Its expression varies in different tissues which can influence its function across multiple biological contexts.
Biological function summary
The MID1 protein serves as an E3 ubiquitin ligase contributing to protein degradation processes. As part of the multiprotein complex MID1 controls the stability of microtubules and interacts with other cytoplasmic proteins. It regulates the turnover of the protein PP2An important for maintaining cellular balance by tagging it for degradation. This function ensures proper cellular morphology and signaling.
Pathways
MID1 influences significant cellular pathways involving the regulation of microtubule dynamics and the degradation of PP2A. It engages in pathways that include the ubiquitin-proteasome system and interacts with proteins linked to these pathways such as alpha4. The interaction with alpha4 suggests a regulatory role in cellular growth and signaling therefore affecting processes like cell cycle control.
MID1 has direct links to conditions such as X-linked Opitz G/BBB syndrome and certain cancers. The dysfunction of MID1 often due to mutations can disrupt normal developmental processes leading to Opitz G/BBB syndrome where it is associated with proteins like PP2A. Similarly alterations in MID1 activity contribute to oncogenesis through its dysregulated participation in protein degradation pathways affecting cellular homeostasis and signaling networks.