Abcam, ab70796, Anti-Cbx8 antibody

Size: 100µL
Rabbit Polyclonal CBX8 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CBX8.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human CBX8. The exact immunogen used to generate this antibody is proprietary information.Q9HC52

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab70796 was affinity purified using an epitope specific to Cbx8 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: PBS, 1.815% Tris, 1.764% Sodium citrate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cbx8 also known as Chromobox protein homolog 8 is part of the Polycomb group proteins with a molecular mass of approximately 50 kDa. Cbx8 functions as a transcriptional regulator by binding to histones and repressive chromatin states. It has a broad expression including in embryonic stem cells and various adult tissues. Its involvement extends to chromatin modification and impacts gene expression regulation making it significant in controlling cellular identity and differentiation.
Biological function summary
Cbx8 contributes to the regulation of gene silencing within the Polycomb Repressive Complex 1 (PRC1). It plays an important role in maintaining cellular memory by ensuring heritable transcriptional repression of target genes. Cbx8's involvement in histone modification particularly histone H3 lysine 27 trimethylation (H3K27me3) is a part of its mechanism to regulate gene expression. This regulation affects key developmental processes and signals cellular differentiation pathways critical for tissue development and maintenance.
Pathways
Cbx8 engages in the Polycomb pathway where it interacts with other PRC1 proteins playing a role in chromatin compaction and silencing of developmental genes. It associates with pathways involved in cell cycle regulation and differentiation linking to essential proteins like RING1 and BMI1. These interactions enable Cbx8 to influence the transcriptional repression necessary for proper execution of developmental programs and cellular response to external signals.
Cbx8 has affiliations with cancer and developmental disorders due to its role in gene silencing. Abnormal Cbx8 activity may lead to disruptions in normal cell cycle controls contributing to oncogenesis with other PRC1 members like BMI1. It also connects to certain developmental syndromes where polycomb dysfunction impacts normal tissue development indicating its broader significance in maintaining cellular homeostasis and genomic stability.