Abcam, ab70843, Anti-MIS12 antibody

Size: 100µL
Rabbit Polyclonal MIS12 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human MIS12.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-P, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MIS12. The exact immunogen used to generate this antibody is proprietary information.Q9H081

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: PBS, 1.815% Tris, 1.764% Sodium citrate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MIS12 also known as MIND kinetochore complex component is a protein primarily involved in chromosome segregation during cell division. The human MIS12 protein has a mass of approximately 22 kDa. It is expressed in proliferating cells especially during mitosis and is a cytoplasmic protein that localizes to the kinetochore an important structure on chromosomes. MIS12 plays a direct role in the stabilization of spindle microtubules to kinetochores ensuring proper chromosome alignment and segregation.
Biological function summary
This protein functions as part of the MIS12 complex which is essential for accurate cytokinesis. The complex is a critical part of the kinetochore-microtubule attachments necessary for mitotic spindle assembly and chromosome movement. MIS12 also interacts with other kinetochore proteins such as NDC80 and DSN1 forming a network that acts in the early mitotic phase of the cell cycle.
Pathways
MIS12 participates in the Spindle Assembly Checkpoint (SAC) a regulatory mechanism ensuring error-free chromosome segregation. It integrates into the mitotic checkpoint signaling pathway interacting with proteins like BUB1 and MAD2 which monitor attachment and tension at kinetochores. This interaction halts progression through mitosis if incorrect attachments are detected preventing chromosomal instability.
Disruptions in the MIS12 protein function are linked to cancers particularly those exhibiting chromosomal instability such as colorectal cancer. MIS12 mutations or misexpression can cause defective chromosome segregation contributing to aneuploidy and tumorigenesis. Additionally its interaction with the APC/C complex important for cell cycle exit connects it to E2F deregulation in cancerous cells.