Abcam, ab7164, Anti-Dystrophin antibody [MANDRA1]

Size: 50µL
Anti-Dystrophin antibody [MANDRA1] (ab7164) is a mouse monoclonal antibody detecting Dystrophin in Western Blot, IHC-Fr, ICC/IF, ELISA . Suitable for Human . - Over 20 publications - Trusted since 2001
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:MANDRA1,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:ELISA, IHC-Fr, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human DMD aa 3200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P11532,
Epitope:128 amino acids at the end of the C-terminal domain of the human dystrophin molecule (a.a. residues 3558-3684).

Product details:
What is this antibody validated in?
Anti-Dystrophin antibody [MANDRA1] (ab7164) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human samples.
Trusted by the scientific community
Anti-Dystrophin [MANDRA1] (ab7164) was first used in a scientific publication in 2001 and has been cited over 20 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purification technique-Proprietary technique, Purification notes-Purified from culture supernatant of hybridoma cells with proprietary method., Storage buffer-Preservative: 0.0975% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Dystrophin also known as the DMD protein plays a mechanical role in muscle fibers by connecting the cytoskeleton of a muscle fiber to the surrounding extracellular matrix through the cell membrane. This structural connection helps reinforce the muscle fiber during contraction and mechanical stress. The protein has a molecular weight of approximately 427 kDa. It is expressed mainly in skeletal and cardiac muscles where it is important for maintaining muscle integrity.
Biological function summary
Dystrophin acts as an important component of the dystrophin-glycoprotein complex. This complex stabilizes the muscle cell membrane by linking actin filaments within the cytoskeleton to proteins in the extracellular matrix. The absence or malfunctioning of dystrophin disrupts this connection leading to increased susceptibility to damage during muscle contraction. This is especially evident in tissues where the protein is abundantly present.
Pathways
Dystrophin is integral to the structural integrity pathway in muscle cells. It works alongside proteins like dystroglycan and sarcoglycan forming a multiprotein complex that ensures cell membrane stability during muscle contractions. The proper functioning of the dystrophin complex is also linked to calcium signaling pathways highlighting its role in cellular signaling mechanisms.
Dystrophin's malfunction is directly associated with Duchenne Muscular Dystrophy (DMD) and Becker Muscular Dystrophy (BMD). Mutations in the DMD gene which encodes the dystrophin protein result in the absence or reduced functionality of the protein leading to progressive muscle degeneration observed in DMD and BMD. These disorders frequently involve the protein utrophin which sometimes compensates for the lack of functional dystrophin albeit insufficiently to alleviate the symptoms.