Abcam, ab71810, Anti-Pneumolysin antibody [PLY-4]

Size: 100µg / 1mg
Mouse Recombinant Monoclonal Pneumolysin antibody. Suitable for WB and reacts with Recombinant full length protein - Streptococcus pneumoniae samples. Cited in 19 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:PLY-4,
Isotype:IgG1,
Carrier free:No,
Reacts with:Streptococcus pneumoniae,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
The production method has been changed from hybridoma to recombinant on 25th Nov 2025.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pneumolysin also known as Ply or ply-004 is a toxin protein produced by Streptococcus pneumoniae a bacterium responsible for many infections. It has a molecular mass of approximately 53 kDa. Pneumolysin is a cholesterol-dependent cytolysin meaning that it targets and binds to cholesterol in host cell membranes leading to pore formation and cell lysis. The toxin is predominantly expressed in S. pneumoniae where it plays an essential role in pathogenicity.
Biological function summary
Pneumolysin affects host cells by disrupting cellular integrity and immune responses. It is not part of a larger protein complex. Pneumolysin can inhibit cilia movement in the respiratory tract impairing the host’s ability to clear mucus and pathogens. This toxin also activates the host immune system by promoting the release of pro-inflammatory cytokines which can result in tissue damage.
Pathways
Pneumolysin plays a significant role in the inflammatory response and cell apoptosis pathways. Within the inflammatory pathway it can trigger the host's immune cells to release cytokines such as interleukin-6 and tumor necrosis factor-alpha. This response leads to increased vascular permeability and leukocyte migration. Pneumolysin-mediated cell lysis also contributes to apoptosis impacting cell survival. The toxin's action relates it to proteins involved in the immune signaling cascades.
Pneumolysin plays a vital role in diseases such as pneumonia and meningitis both caused by S. pneumoniae. The disruption of host cell membranes and induction of inflammation contribute directly to disease symptoms. Pneumolysin interacts with proteins involved in the host's immune defense during infection. These interactions and its cytotoxic properties make pneumolysin a target for research into vaccine and therapeutic strategies against pneumococcal diseases.