Abcam, ab72083, Anti-DDX46 antibody

Size: 100µL
Rabbit Polyclonal DDX46 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human DDX46.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human DDX46. The exact immunogen used to generate this antibody is proprietary information.Q7L014

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DDX46 also known as DEAD-box helicase 46 is a protein involved in RNA processing and is part of the DEAD-box protein family. It has a molecular mass of approximately 103 kDa. Mechanically DDX46 functions as an ATP-dependent RNA helicase unwinding RNA secondary structures. This action is essential during RNA splicing events. DDX46 is expressed in various tissues including the liver testes and brain pointing to its importance in diverse cellular functions.
Biological function summary
DEAD-box helicases like DDX46 play a role in the biogenesis of ribosomal RNA and pre-mRNA splicing. DDX46 specifically contributes to the assembly of the U4/U6.U5 tri-snRNP complex a major component of the spliceosome. The spliceosome is a large and dynamic molecular complex important for the splicing of precursor messenger RNA transforming it into mature messenger RNA. DDX46's helicase activity facilitates RNA-RNA base pairing changes necessary for spliceosome assembly.
Pathways
DDX46 acts within the RNA splicing pathway coordinating with other spliceosomal proteins such as PRPF31 and SNRNP200. These proteins work together to ensure accuracy and efficiency in the removal of introns from pre-messenger RNA. DDX46 has a role in the regulation of gene expression and post-transcriptional gene regulation. It indirectly contributes to the signal transduction processes by affecting the maturation of mRNAs coding for proteins involved in important pathways.
Disruptions in DDX46 function have been linked to inherited retinal diseases and hematological disorders. DDX46 mutations or malfunctions can be associated with retinitis pigmentosa a disorder often connected with proteins such as PRPF8 which is another spliceosomal component. Moreover abnormalities in mRNA splicing due to impaired DDX46 can lead to myelodysplastic syndromes disorders characterized by ineffective hematopoiesis and often associated with mutations in spliceosome genes.